| 补肾法对卵巢切除大鼠骨组织白细胞介素-6mRNA表达的影响 |
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| 引用本文： | 沈霖,杜靖远,涂意辉,杨艳萍,谢晶,周丕琪. 补肾法对卵巢切除大鼠骨组织白细胞介素-6mRNA表达的影响[J]. 中国骨伤, 2002, 15(4): 211-213 |
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| 作者姓名： | 沈霖 杜靖远 涂意辉 杨艳萍 谢晶 周丕琪 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院,湖北,武汉,430022 |
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| 基金项目： | 国家中医药管理局课题(编号2000-J-B-19) |
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| 摘 要： | 目的探讨补肾法治疗绝经后骨质疏松症的分子作用环节.方法以卵巢切除大鼠作为骨质疏松实验模型,通过斑点杂交技术,在mRNA水平分析模型组、密骨片组及假手术组白细胞介素-6(IL-6)表达.通过原位杂交技术,研究了IL-6 mRNA在骨组织细胞的分布.结果模型组大鼠IL-6 mRNA表达较假手术组明显升高(P＜0.01);而密骨片组治疗后IL-6 mRNA表达明显下降(P＜0.01).IL-6 mRNA主要在成骨细胞、骨衬里细胞和骨细胞胞浆内表达.结论 IL-6在骨质疏松骨丢失过程中起重要作用;补肾法抗骨质疏松疗效部分是通过降低IL-6产生而介导的.
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| 关 键 词： | 骨质疏松 绝经后 卵巢切除术 中医现代化 治疗 |
| 收稿时间： | 2001-10-15 |
| 修稿时间： | 2001-10-15 |
Effects of kidney invigoration method on expression of interleukin-6 mRNA in bone of ovariectomized rats |
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| SHEN Lin,DU Jing-yuan,TU Yi-hui. Effects of kidney invigoration method on expression of interleukin-6 mRNA in bone of ovariectomized rats[J]. China journal of orthopaedics and traumatology, 2002, 15(4): 211-213 |
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| Authors: | SHEN Lin DU Jing-yuan TU Yi-hui |
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| Affiliation: | Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022;Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022;Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022;Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022;Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022;Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| Abstract: | Objective:investigate the molecular links of the treatment with kidney invigoration method on postmenopausal osteoporosis in vivo. Methods:Adult ovariectomized rats were used in this study as the model of osteoporosis. Expression of interleukin-6(IL-6)in bone tissue of sham operate(Sham) .ovariecto-my(OVX)and ovariectomy with Migu tablet(OVX + MGT)rats was analysed at mRNA level using dot blot hybridization. The distribution of IL-6 mRAN was studied by in situ hybridization. Results:The expression of IL-6 was higher in bone tissue of OVX than that of Sham group(P<0.01) .while treated with MGT.the ex-pression of IL-6 significantly declined compared with that in OVX group(P<0.01).The in situ hybridization showed that 11-6 mRNA was mainly expressed in osteoblasts, lining cell and osteocytes. Conclusion: These date indicate that IL-6 may pky an important role in bone loss of osteoporosis. Suppression for IL-6 production was involved in the mechanism of kidney invigoration in the treatment of osteoporosis. |
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| Keywords: | Osteoporosis,postmenopausal Ovariectomy Moderation of TCM |
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