5-氟尿嘧啶联合蒽贝素治疗结肠癌的研究

目的：探讨5-氟尿嘧啶（5-FU）联合蒽贝素对结肠癌的治疗作用。方法：MTT法和肿瘤球生长实验考察5-FU联合蒽贝素对结肠癌C26细胞的毒性。建立结肠癌小鼠移植瘤模型，随机分为生理盐水组、5-FU组、蒽贝素组和联合给药组。各组小鼠均3d一次尾静脉注射给药。观察给药过程中瘤体体积的变化，考察不同给药组对小鼠肿瘤生长的抑制效果。结果：给药48h后生理盐水组肿瘤细胞存活率为95％，5-FU组、蒽贝素组和联合给药组肿瘤细胞存活率分别为48％、69％和18％，与单独给药组相比，联合给药能够显著增强对C26细胞的增殖抑制作用（P〈0．01）。给药7d后，生理盐水组肿瘤球持续生长，体积增大1．52倍，3个给药组肿瘤球体积出现了不同程度的减小，5-FU组、蒽贝素组和联合给药组分别使肿瘤体积减小到原体积的71％、89％和33％（P〈0．01）；生理盐水组、蒽贝素组、5-FU小鼠肿瘤体积成长分别变为给药前肿瘤体积的1．85倍、1．79倍和1．49倍，而联合给药组为1．21倍（P〈0．01）。联合给药组与蒽贝素组和5-FU组单独给药组间差异具有统计学意义（P〈0．01）。同时联合给药能够延长荷瘤小鼠的中位生存期。结论：5-FU与蒽贝素都具有抗结肠癌作用．且二者联合使用效果更显著。

Effects of 5-FU combined with embelin for the treatment of colorectal cancer

AIM. To evaluate the effect of 5- FU combined with embelin on the treatment of colorectal cancer. METHODS： The anti-proliferation efficiency of 5-FU combined with embelin was evaluated by MTT assay and colony-forming assay. C26 cells were xenografted in mice to establish the animal model, which were used to evaluate the effect of anti-tumor. The tumor size sere measured every group. HE were used to observe the apoptosis of cancer cells. RESULTS： Both 5-FU and embelin could inhibit the growth of C26 cells compare to saline. The cell viability of C26 cells were 95%, 48%, 69% and 18% after the treatment of saline, 5-FU, embelin and 5-FU ＋ embelin, respectively （P〈 0.01 ）. Tumor spheroids continued to grow in size and volume in saline 152% of the primary volume.The change ratios of tumor spheroid volumes （%） were nearly 71%, 89% and 33% for 5-FU, embelin and 5-FU＋ embelin, respectively （P 〈0.01）. The inhibition of colorectal cancer in vivo demonstrated the change ratios of tumor volumes （%） were nearly 179%, 149% and 121% for 5-FU, embelin and 5-FU ＋ embelin, while the saline group was 185%, respectively （P〈 0.01）. CONCLUSION： The 5-FU and embelin can inhibit the growth of colorectal cancer, and the combination of 5-FU and embelin may be a potentially effective treatment for colorectal cancer.