地奥司明对肾缺血/再灌注大鼠氧化应激及肾功能的影响

目的观察地奥司明(diosmin,DOSM)对大鼠肾缺血/再灌注(ischemia/resperfusion,I/R)肾组织中氧化应激水平及肾功能的影响。方法 180只SD大鼠随机分为3组:假手术组(sham operation,SO)、I/R模型组和DOSM+I/R组。采用ELISA方法测定肾组织中丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)含量的变化,同时检测血肌酐(creatinine,Cr)和尿素氮(blood urea nitrogen,BUN)水平。结果在缺血再灌注后1h、3h、6h和12h肾组织中MDA含量逐渐升高,12h达到高峰,I/R组和DOSM组均显著高于SO组(P<0.01);此后MDA逐渐下降,DOSM组血清MDA水平显著低于I/R组(P<0.01)。随再灌注时间的延长,肾组织匀浆中SOD活性显著降低,并于24h达最低水平,此后开始回升。DOSM组在6h、12h、24h和48h与I/R组相比不同程度地提高SOD的活力(P<0.01或P<0.05),与SO组相比无统计学差异。血Cr和BUN水平在DOSM+I/R组显著低于I/R组(P<0.05或P<0.01)。结论大鼠肾I/R可以引起肾组织的脂质过氧化反应增强,DOSM可通过抗氧自由基损伤及减轻脂质过氧化反应而保护肾功能。

Effect of diosmin on oxidative stress and renal function in rats after ischemia/reperfusion injury

Objective To observe the effect of diosmin on oxidative stress and renal function in rats after ischemia/reperfusion（I/R） injury.Methods One hundred and eighty SD rats were randomly divided into sham operation（SO） group,I/R injury model group,and diosmin ＋I/R injury group.Serum levels of malonaldehyde（MDA）,superoxide dismutase（SOD）,creatinine（Scr）,and blood urea nitrogen（BUN） in their kidney tissue were measured by ELISA.Results The serum MDA level increased gradually in kidney tissue of rats at 1,3,6 and 12h after I/R injury and reached its peak at 12h,and was significantly higher in I/R injury model group and diosmin ＋I/R injury group than in sham operation group（P0.01）.Then,the serum MDA level decreased gradually and was significantly lower in diosmin＋I/R injury group than in I/R injury group（P0.01）.The SOD activity decreased significantly in renal tissue of rats,reached its maximum at 24 after I/R injury,then began to increase,and was significantly higher in diosmin ＋I/R group than in I/R injury group at 6,12,24 and 48h after I/R injury（P0.01 or P0.05）.However,no significant difference was found in SOD activity between diosmin ＋I/R injury group and sham operation group.The serum Scr and BUN levels were significantly lower in diosmin ＋I/R injury group than in I/R injury group after I/R injury（P0.01 or P0.05）.Conclusion I/R can increase lipid peroxidation in renal tissue of rats.Diosmin can protect renal function by reducing its anti-free radical injury and alleviating its lipid peroxidation.