| 环丙沙星特异性抗体的研制 |
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| 引用本文： | 李平,邓省亮,刘洪斌,于洪侠,邱静,杨曙明.环丙沙星特异性抗体的研制[J].细胞与分子免疫学杂志,2011,27(1):64-67. |
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| 作者姓名： | 李平 邓省亮 刘洪斌 于洪侠 邱静 杨曙明 |
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| 作者单位： | 1. 中国农业科学院,农业质量标准与检测技术研究所,北京,100081
2. 中国农业科学院,农业质量标准与检测技术研究所,北京,100081;江西省科学院微生物研究所,江西,南昌,330029 |
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| 基金项目： | 国家高技术研究发展计划(863)资助项目 |
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| 摘 要： | 目的:制备抗环丙沙星(CIP)的特异性抗体,以ELISA方法对其进行评价,为进一步研制CIP快速检测试剂盒打基础。方法:以阳离子化牛血清白蛋白(cBSA)为载体合成免疫原CIP-cBSA,免疫新西兰长耳白兔和BALB/c小鼠,同时以天然、阳离子化鸡卵清白蛋白(nOVA、cOVA)为载体合成包被原CIP-nOVA、CIP-cOVA进行抗血清的筛选,以间接(竞争)ELISA法测定抗血清的效价及特异性。结果:本试验获得7种抗血清,其中2种高特异性CIP抗血清,IC50达1μg/L,效价分别为4000和2000(以A450值为1.0时的抗血清稀释倍数表示),两种抗血清与恩诺沙星(ENR)、诺氟沙星(NOR)、氧氟沙星(OFL)存在不同程度的交叉反应,与青霉素(PEN)、卡那霉素(KAN)和庆大霉素(QEN)无交叉。结论:可满足目前中国动物性食品安全检测的需要,为研究组织中CIP残留及开发CIP快速检测试剂盒奠定了基础。
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| 关 键 词： | 环丙沙星 特异性抗体 ELISA |
Development of specific antibodies against ciprofloxacin |
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| LI Ping,DENG Sheng-liang,LIU Hong-bin,YU Hong-xia,QIU Jing,YANG Shu-ming.Development of specific antibodies against ciprofloxacin[J].Journal of Cellular and Molecular Immunology,2011,27(1):64-67. |
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| Authors: | LI Ping DENG Sheng-liang LIU Hong-bin YU Hong-xia QIU Jing YANG Shu-ming |
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| Institution: | LI Ping1,DENG Sheng-liang1,2,LIU Hong-bin1,YU Hong-xia1,QIU Jing1,YANG Shu-ming1 1Institute of Quality Standard and Testing Technology for Agro-Product,Chinese Academy of Agriculture Sciences,Beijing 100081,2Institute of Microbiology,Jiangxi Province Academy of Sciences,Nanchang 330029,China |
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| Abstract: | AIM:To prepare specific antibodies against ciprofloxacin (CIP) and characterize the antibodies properities with ELISA, then lay the foundation for development of CIP rapid detection kit. METHODS: New Zealand white rabbits and BALB/c mice were immunized with CIP-cBSA conjugate, CIP-nOVA and CIP-cOVA serving as coating antigen were used to screen antisera, indirect (competitive) ELISA was developed to determine the titer and specificity of the antisera. RESULTS: Seven antisera against CIP were obtained, two o... |
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| Keywords: | ELISA |
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