胎肝前体细胞脾移植对大鼠急性肝功能衰竭的实验研究

目的 :探讨经脾内同种异体移植培养的原代胎肝前体细胞与成体肝细胞悬液对大鼠药物性肝衰竭的疗效 ,并观察脾内移植肝细胞的生物学特性。方法 :采用 D-氨基半乳糖 ( D- gal)建立大鼠急性肝衰竭模型 ,2 4 h后随机分为三组进行治疗。 A组 :经脾内移植体外培养 7d的肝细胞 2× 1 0 7;B组 :经脾内移植肝细胞悬液 2× 1 0 7;C组 :经脾内注射生理盐水1 ml。观察受体大鼠的存活率、肝脏功能和病理变化及移植肝细胞的生物学特性。结果 :A组、B组大鼠存活率 ( 77%、5 9% )与 C组大鼠存活率 ( 2 2 % )相比具有显著性差异 ,肝功能各项指标均有明显改善 ,A、B组与 C组大鼠的肝功能改变方面有统计学差异。经 HE和 PAS染色证实 ,移植的肝细胞在受体脾内结构和功能保持较好。结论 :经脾内移植的培养的原代胎肝前体细胞和肝细胞悬液均能提高大鼠药物性肝衰竭的存活率、改善肝功能及肝脏组织病理变化 ,但培养原代胎肝前体细胞优于成体肝细胞悬液。

Intrasplenic transplantation of hepatic progenitor of fetal liver on rats with acute hepatic failure

Objections: To study the effects of intrasplenic transplantation of primary cult ured he patic progenitor of fetal liver and isolated hepatocytes on rats with acute hepa tic failure (AHF) induced by D-galactosamine (D-gal), and to observe the biolo gical characteristics of transplanted hepatocytes in the spleen.Methods: AHF rat s were induced by D-gal. Hepatocyte transplantation was carried out 24 hours af ter intoxication, and the rats were divided into three groups randomly. In Group A, rats received intrasplenic injection of 2×10 7 hepatic progenitor of fetal l iv er cultured for 7 days, whereas in Group B, rats received 2×10 7 hepatocytes i nt rasplenically. In Group C, rats received 1mL normal saline intrasplenically as c ontrol. Then the survival rates, liver function, liver histology and the biological characteristics of transplanted hepatocytes i n all rats were observed.Results: Compared with Group C , a significant improvem ent in survival rates was observed in Group A and B(77 % vs 22 %, P<0.01 and 59% vs 22 %, P<0.05 respectively).Three days after hepatocyte transplantati on,t he liver functions in Group A and B rats were restored significantly compared wi th those of Group C, and the liver pathologic changes were mild . But no differe nce was found in the survival rates and liver functions between Groups A and B. By the HE and PAS staining, our data indicated that the structure and function o f hepatocytes transplanted kept basically well.Conclusion: Intrasplenic transpl antation of primary cultured progenitor of fetal liver and isolated hepatocytes can prolong the survival of AHF rats and improve the liver function and histolo gy, and no statistically significant differences are observed between Group A an d B. Our data suggest that progenitor of fetal liver may be an alternative for h epatocyte transplantation.