Influence of cations on the electrical activity of neuroblastoma × glioma hybrid cells |
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Authors: | Georg Reiser Rolf Heumann Wayne Kemper Edith Lautenschlager Bernd Hamprecht |
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Institution: | aMax Planck Institut fu¨r Biochemie, 8033 Martinsried, G.F.R. |
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Abstract: | Electrical excitability is one of the various neuronal properties of neuroblastoma × glioma hybrid cells. At a Ca2+ concentration of 1.8mM the action potential is inhibited by tetrodotoxin, suggesting that the inward current is carried by Na+ ions. In contrast, at a Ca2+ concentration of 20–36mM and even in the absence of Na+, spikes (sometimes repetitive) with strong hyperpolarizing afterpotential occur, which are no longer affected by tetrodotoxin. They are, however, blocked by antagonists of Ca2+ like La3+, Co2+, Mn2+, and the synthetic compounds D-600 and BAY a-1040. This seems to indicate that at high concentrations of Ca2+, the inward current of the action potential is essentially carried by Ca2+. Sr2+, but not Mg2+ can effectively substitute for Ca2+. It shows down the time course of the action potential. Ba2+ depolarizes the membrane gradually. If Ca2+ is also present, Ba2+ causes a reduced depolarization and spontaneous action potentials with no hyperpolarizing after-potential are observed. |
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