GC法测定鸦胆子油口服乳液中油酸的含量

目的 建立鸦胆子油口服乳液中油酸的含量测定方法.方法 采用气相色谱(GC)法,色谱柱为极性毛细管柱(DB-WAX 30 m×0.25 mm,0.25 μm)；检测器:FID；柱温程序升温:起始温度180℃,以每分钟10℃升至200℃,保持1 min,再以每分钟10℃升至220℃,保持10 min.结果 油酸在0.233～2.370 μg范围内具有良好的线性关系(r=0.99998),油酸的平均回收率为98.6％,RSD为2.5％.结论 该方法简便准确,重现性好,专属性强,可用于鸦胆子油口服乳液的质量控制.

Determination of Oleic Acid in Fructus Bruceae Oil Oral Emulsion by Gas Chromatography

Objective To establish an gas chromatography(GC) method for the determination of oleic acid in Fructus Bruceae Oil Oral Emulsion.Methods GC was equipped with FID detector,and a DB-WAX(3.0 m×0.25 mm,0.25 μm) capillary column was used.The temperature program of column oven was as follows:initial temperature at 180 ℃,increasing the temperature at a rate of 10 ℃·min-1 to 200 ℃,maintaining for 1 min,then increasing at a rate of 10 ℃·min-1 to 220 ℃,maintaining for 10 min.Results The results showed a good linearity(r=0.99998) of oleic acid between 0.233～2.370 μg.The average recovery was 98.6 % and RSD 2.5 %.Conclusion The method is simple,accurate and specific.It can be used for the quality control of Fructus Bruceae Oil Oral Emulsion.