比较含黄芪和含红芪的补中益气汤对小鼠免疫功能的影响

目的 探讨黄芪和红芪在补中益气汤提高免疫功能作用过程中的可替性.方法 取正常昆明种小鼠40只,随机分为对照组、模型组、含黄芪组和含红芪组,每组10只.腹腔注射环磷酰胺制备免疫功能低下的小鼠模型.含黄芪组和含红芪组分别给予含黄芪和红芪的补中益气汤20ml/kg,连续14d,对照组和模型组给予等量生理盐水.测定各组小鼠胸腺、脾脏质量并计算小鼠胸腺指数、脾脏指数；检测巨噬细胞吞噬功能、血清溶血素水平、T淋巴细胞增殖能力、自然杀伤细胞(NK)杀伤活性及白细胞介素4(IL-4)含量. 结果 与模型组相比,含黄芪组和含红芪组均可提高免疫抑制小鼠胸腺指数、脾脏指数、巨噬细胞吞噬指数、溶血素水平、T细胞增殖能力、NK细胞活性、IL-4水平(P＜0.05或P＜0.01).除含红芪组提高T细胞增殖能力作用强于含黄芪组外,其余免疫指标两组间比较差异无统计学意义(P＞0.05).结论 在提高免疫功能方面,补中益气汤中红芪和黄芪是可以相互替换的.

Comparison of Effect of Buzhong Yiqi Decoction Containing Astragalus Membranaceus and Containing Radix Hedysari on Immunologic Function of Mice

Objective To study the substitutablility of Huangqi (Astragalus membranaceus) and Hongqi (Radix hedysari) in Buzhong Yiqi Decoction (Decoction for reinforcing middle-jiao and replenishing qi) in improving immunologic function in mice. Methods Forty normal Kunming mice were randomized into four groups with 10 in each,i.e. control group, cyclophosphamide model group, BuzhongYiqi Decoction containing Huangqi group (Huang qi group), and Buzhong Yiqi Decoction containing Hongqi group (Hongqi group). The mice were injected peritoneally with cyclophosphamide to establish the immunosuppressive models. Huangqi group and Hongqi group were given Buzhong Yiqi Decoction 20ml/kg containing Huangqi and Hongqi respectively for 14 days, while the control group and model group were given normal saline of equal volume. The thymus and spleen weights were measured to calculate the thymus and spleen indices, the phagocytic function of macrophages, serum hemolysin, T lymphocyte proliferation, the activity of NK cells, and the IL-4 were determined of all the above-mentioned indexes (P<0.05 or P<0.01). Results Comparing with the model group, both BuzhongYiqi Decoction groups were found increase. Except that the T lymphocyte proliferation of the Buzhong Yiqi Decoction containing Hongqi group was stronger than that of the BuzhongYiqi Decoction containing Huangqi group, the other immunologic indices of the two BuzhongYiqi Decoction groups were not statistically different (P>0.05). Conclusion In improving the cellular immunologic function, Hongqi and Huangqi can replace each other in Buzhong Yiqi Decoction.