| MTT法检测没食子酸对两种细胞的体外毒性 |
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| 引用本文： | 严晓莺,陈巨鹏,王明艳.MTT法检测没食子酸对两种细胞的体外毒性[J].中医药信息,2012,29(2):107-109. |
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| 作者姓名： | 严晓莺 陈巨鹏 王明艳 |
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| 作者单位： | 1. 南京中医药大学,江苏南京,210046
2. 江苏省中医院,江苏南京,210029 |
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| 基金项目： | 江苏省中医药药效与安全评价重点实验室开放课题 |
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| 摘 要： | 目的:探讨抗肿瘤单体没食子酸(GA)对体外培养的正常人肝细胞株LO2和肝癌细胞株SMMC-7721的毒性。方法:采用MTT实验分析GA对LO2和SMMC-7721的增殖抑制作用。结果:GA作用24h、48h、72h后,用药组均出现了不同程度的增殖抑制。结论:抗肿瘤单体GA对LO2和SMMC-7721细胞均有一定的毒性,表现为增殖抑制作用,作用早期(24h)对LO2的抑制大于对SMMC-7721的抑制,连续作用后(48h、72h),对SMMC-7721细胞的损伤明显超过对LO2的抑制。
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| 关 键 词： | 没食子酸 肝细胞 肝癌细胞 MTT |
Determination of Gallic AciLd on In - vitro Toxicity of Two Kinds of Cells by MTT |
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| YAN Xiao-ying
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CHEN Ju-peng
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WANG Ming-yan.Determination of Gallic AciLd on In - vitro Toxicity of Two Kinds of Cells by MTT[J].Information on Traditional Chinese Medicine,2012,29(2):107-109. |
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| Authors: | YAN Xiao-ying CHEN Ju-peng WANG Ming-yan |
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| Institution: | YAN Xiao - ying, CHEN Ju - peng, WANG Ming -yan ( 1. Nanjing University of Chinese Medicine, Nanjing 210046, China ; 2. Jiangsu Piovincal TCM Hospital, Nanjing 210029, China) |
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| Abstract: | Objective ： To investigate tile toxicity of anti - tumor monomer gallic acid （GA） on normal human hepacocyte strain LO2 and hepatocarcinoma cell strain SMMC -7721 cuhivated in vitro. Methods： MTF ex- periment was adopted to analyze the inhibitory effects of GA on the proliferation of LO2 and SMMC -7721. Results ： Medication groups respectively presented different degrees of proliferation inhibition after GA acted on 24h, 48h, and 72h. Conclusion： Anti - tumor monomer GA has certain toxicity on both cells LO2 and SMMC -7721, manifesting proliferation inhibitory effects, namely the inhibition on LO2 is stronger than that on SMMC -7721 at the early action （24h） while on SMMC -7721 cell injury significantly more than on the proliferation of LO2 after continuous action （48h, 72h）. |
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| Keywords: | Gallic acid （GA） Hepatocyte Hepatocarcinoma cell MTT |
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