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| 莱菔子素对人肝细胞Ⅱ相代谢酶的作用研究 | |
| 引用本文： | 潘萌,黄睿,潭洁,李宝龙,冯雪,王凤前,单毓娟. 莱菔子素对人肝细胞Ⅱ相代谢酶的作用研究[J]. 中医药信息, 2012, 29(2): 13-15 |
| 作者姓名： | 潘萌 黄睿 潭洁 李宝龙 冯雪 王凤前 单毓娟 |
| 作者单位： | 1. 哈尔滨医科大学公共卫生学院,黑龙江哈尔滨,150081 2. 哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081;杭州市妇女医院,浙江杭州310016 3. 黑龙江中医药大学,黑龙江哈尔滨,150040 4. 哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081;哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090 |
| 摘 要： | 目的:探讨莱菔子素(sulforaphene)对人肝细胞系HHL-5细胞Ⅱ相代谢酶的影响。方法:采用噻唑蓝(MTT)比色法测定莱菔子素对HHL-5细胞的毒性作用;蛋白印记法(western blotting)检测莱菔子素对Ⅱ相代谢酶蛋白表达的影响。结果:5μmol/L莱菔子素处理HHL-5细胞24h促进细胞增殖,10～100μmol/L表现生长抑制作用;莱菔子素能明显诱导血红素加氧酶(HO-1)和硫氧还蛋白还原酶(TrxR)蛋白的表达并呈剂量依赖关系。结论:莱菔子素可诱导肝细胞Ⅱ相代谢酶HO-1和TrxR的表达,具有潜在的化学致癌物解毒功能。 |
| 关 键 词： | 莱菔子素 莱菔硫烷 血红素加氧酶-1 硫氧还蛋白还原酶 |
Induction of Phase H Metabolic Enzymes by Sulforaphene in Human Immortalized Hepatocytes |
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| PAN Meng , HUANG Rui , TAN Jie , LI Bao-long , FENG Xue , WANG Feng-qian , SHAN Yu-juan. Induction of Phase H Metabolic Enzymes by Sulforaphene in Human Immortalized Hepatocytes[J]. Information on Traditional Chinese Medicine, 2012, 29(2): 13-15 | |
| Authors: | PAN Meng HUANG Rui TAN Jie LI Bao-long FENG Xue WANG Feng-qian SHAN Yu-juan |
| Affiliation: | PAN Meng,HUANG Rui ,TAN Jie,LI Bao - long ,FENG Xue ,WANG Feng - qian ,SHAN Yu -juan ( 1. Harbin Medical University, Harbin 150081, China ; 2. Hangzhou Women＇s Hospital, Hangzhou 31 O016, China ; 3. Heiloriang University of Chinese Medicine ,Harbin 150040, China ;4. Harbin Institute of Technology ,Harbin 150090, China) |
| Abstract: | Objective ： To study the effect of sulforaphene on phase Ⅱ metabolic enzymes expressions in human immortalized hepatoeytes （HHL -5 cells）. Methods： MTY assay was used to test the cytotoxicity of sulfora- phene. HO - 1 and TrxR expressions were detected by western blot analysis. Results： Sulforaphene promoted HHL -5 cells growth at 5μmol/L and inhibited cells growth at 10 - 100μmoL/L. Moreover, HO - 1 and TrxR proteins were up - expressions of HO cinogen. regulated in a dose -dependent manner at 24h. Conclusion： Sulforaphene can induce the -1 and TrxR proteins ,which are attributed to the detoxification functions of chemical car-cinogen. |
| Keywords: | Sulforaphene Sulforaphane Heme oxygenase - 1 Thioredoxin reductase |
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