晶状体上皮细胞(LECs)凋亡及凋亡相关基因caspase-3表达与老年性白内障

目的 探讨晶状体上皮细胞(LECs)凋亡及凋亡相关基因caspase-3表达与老年性白内障发生的关系.方法 选择32例老年性白内障患者晶状体前囊膜标本(白内障组),同时收集高度近视患者晶状体前囊膜标本28例(高度近视组).采用免疫组化法检测两组的晶状体前囊膜组织中凋亡相关基因caspase-3的表达,并应用TUNEL法检测两组LECs的凋亡情况.结果 白内障组LECs中凋亡细胞百分率(30.4±3.7)％,明显高于高度近视组LECs中凋亡细胞百分率(0.27±0.12)％],差异有统计学意义(P＜0.05).白内障组LECs中caspase-3蛋白的阳性表达率为84.38％,明显高于高度近视组LECs中caspase-3蛋白的阳性表达率(10.71％),差异有统计学意义(P＜0.05).等级Logistic回归分析显示:老年性白内障患者的LECs中caspase-3蛋白的表达明显高于高度近视组(P＜0.01).结论 LECs的凋亡及caspase-3的表达在老年性白内障的发生发展过程中起重要作用.

ANALYSIS OF THE RALATIONSHIP BETWEEN LENS EPITHELIAL CELLS(LECs) APOPTOSIS OR APOPTOSIS RELATED GENE caspase-3 EXPRESSION AND SENILE CATARACT

Objective To explore the relationship between lens epithelial cells(LECs)apoptosis or apoptosis related gene caspase-3 expression and senile cataract.Methods The specimen from anterior lens capsule membrane in 32 cases of patients with senile cataract(cataract group)were taken.Meanwhile,the specimen from anterior lens capsule membrane in 28 cases of high myopia(high myopia group)were also colected.Using immunohistochemical method to detect apoptosis related gene caspase-3 expression in anterior lens capsule membrane tissue from both groups and the TUNEL method was used to detect the LECs apoptosis situation in both groups.Results LECs apoptotic cell percentage was 30.4%±3.7 in cataract group and obviously higher than that of in high myopia group 0.27%±0.12,the difference was statistically significant(P<0.05).The positive expression rate of LECs caspase-3 protein was 84.38% in cataract group and that was much higher than the positive expression rate(10.71%) of LECs caspase-3 protein in high myopia group,the difference was statistically significant(P<0.05).Grade Logistic regression analysis showed that the LECs caspase-3 protein expression in senile cataract patients was obviously higher than that of in the high myopia group(P<0.01).Conclusion LECs apoptosis and caspase-3 expression may play an important role in pathogenesis and development of senile cataract.