头针透刺对脑出血大鼠脑组织IL-6表达影响的研究

目的:通过对白介素-6(IL-6)蛋白表达变化的观察,探讨头穴透刺对急性脑出血(Intracere-bral hemorrage,ICH)炎症反应的干预作用。方法:将96只健康雄性Wistar大鼠按完全随机方法平均分为模型组、针刺组和西药组。每组再按照6h、24h、48h、72h和168h分为5个亚组,每个亚组6只大鼠,另设6只大鼠作为空白对照组。制备ICH大鼠模型,采用免疫组化技术观察不同时间点头穴透刺对ICH大鼠脑组织IL-6蛋白表达的影响。结果:针刺组大鼠脑内IL-6表达在不同时间点均有显著下调,与模型组比较具有显著性差异(P0.05)。结论:针刺可抑制IL-6蛋白表达,减轻脑出血后炎症反应所致的继发性脑损伤。

Effect of Acupuncture on the Expression of IL-6 in the Brain Tissue around Hematoma of Rats with Intercerebral Hemorrhage

Objective： To study the dynamic expressions of IL-6 after intracerebral hemorrhage（ICH） rats and the influence of acupuncture on them.Methods： The 96 healthy Wistar male rats were selected.90 healthy male Wistar rats were randomly divided into 3 groups：model group,acupuncture group and western medicine group,30 rats in each group.Each group was divided into 5 time phase point of 6 hours,1day,2days,3days and 7days,respectively,6 rats in each group.Meanwhile,6 rats were taken as the blank control group.The intracerbral hematoma rat model was made by autologous blood injection using stereotactic method.The expression of IL-6 immunohistochemical method were checked.Results： The expressions of IL-6 had an obvious down-regulation at different phases in the brain of the acupuncture group rats,and it showed significant differences compared with model group（P0.05）.Conclusion： The acupuncture of BaiHui-QuBin can antagonize brain edema and secondary brain injury caused by inflammatory response after ICH by means of inhibiting the influence of IL-6 during the acute period of intracerebral hemorrhage.