| 氧化型低密度脂蛋白对单核细胞表达尿激酶型纤溶酶原激活物受体的影响 |
| |
| 引用本文： | 钱 赓,陈 未,陈光辉,等. 氧化型低密度脂蛋白对单核细胞表达尿激酶型纤溶酶原激活物受体的影响[J]. 中华老年多器官疾病杂志, 2011, 10(2): 125-127 |
| |
| 作者姓名： | 钱 赓 陈 未 陈光辉 等 |
| |
| 作者单位： | 解放军总医院心血管内科,北京,100853 |
| |
| 摘 要： | 目的探讨不同浓度的氧化型低密度脂蛋白（oxLDL）对体外培养单核细胞表达尿激酶型纤溶酶原激活物受体（uPAR）的影响。方法采用密度梯度离心法及黏附法分离、提取并纯化健康人外周血单核细胞,对原代培养的单核细胞分别加入25,50,100mg/L浓度的oxLDL,分别培养12,24,48h,测定单核细胞的uPAR蛋白表达量及uPAR mRNA的水平变化。药物干预组加入含阿托伐他汀（终浓度为5.0μmo/L）和50mg/L的ox-LDL的培养液培养12,24,48h,同样方法测定单核细胞的uPAR蛋白表达量及uPAR mRNA的水平变化。结果与正常组比较,oxLDL刺激组单核细胞uPAR蛋白表达水平有显著升高,并呈剂量依赖关系;oxLDL刺激组单核细胞uPAR mRNA的合成量被显著上调。阿托伐他汀干预组uPAR蛋白的表达水平及uPAR mRNA合成量的刺激效果均被显著抑制。结论 oxLDL刺激单核细胞高表达uPAR是通过转录水平的上调来刺激蛋白质合成增加的;阿托伐他汀对uPAR表达的抑制是通过下调uPAR转录水平来实现的。
|
| 关 键 词： | 动脉粥样硬化 单核细胞 尿纤溶酶原激活物 受体 低密度脂蛋白 |
Clinical correlation between serum inflammatory cytokines and acute coronary syndrome |
| |
| QIAN Geng,CHEN Wei,CHEN Guanghui,YI Jun,CHEN Yundai. Clinical correlation between serum inflammatory cytokines and acute coronary syndrome[J]. Chinese Journal of Multiple Organ Diseases in the Elderly, 2011, 10(2): 125-127 |
| |
| Authors: | QIAN Geng CHEN Wei CHEN Guanghui YI Jun CHEN Yundai |
| |
| Affiliation: | QIAN Geng~*,CHEN Wei,CHEN Guanghui,YI Jun,CHEN Yundai (Department of Cardiology,Chinese PLA General Hospital,Beijing 100853,China) |
| |
| Abstract: | Objective To investigate urokinase type plasminogen activator receptor(uPAR) expressions in cultured monocytes after exposure to different concentrations of oxidized low density lipoprotein(oxLDL).Methods Monocytes were isolated from healthy volunteers and purified by density gradient centrifugation and adhering assay.The monocytes were incubated with different concentrations of oxLDL(25.50,and 100 mg/L) for 12,24 and 48 hours.The expressions of uPAR protein and mRNA were detected with ELISA and RT-PCR resp... |
| |
| Keywords: | atherosclerosis monocytes urokinase type plasminogen activator receptor low density lipoprotein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中华老年多器官疾病杂志》浏览原始摘要信息 |
|
点击此处可从《中华老年多器官疾病杂志》下载全文 |
|
