应用磁纳米捕获-PCR技术检测痰液结核分枝杆菌的研究

目的采用磁纳米捕获技术富集痰液中的结核分枝杆菌,以提高PCR检测的灵敏度,快速诊断结核病。方法以普通PCR为对照,采用磁纳米捕获技术富集187份结核和非结核呼吸系统疾病患者痰标本中的结核分枝杆菌,进行PCR检测。结果 152份肺结核患者痰标本41份(27.0%)涂片抗酸染色阳性,72份(47.4%)普通PCR检测阳性,126份(82.9%)磁纳米捕获-PCR检测阳性。35份非结核呼吸系统疾病患者,痰标本中抗酸染色均为阴性,1份普通PCR和磁纳米捕获-PCR检测均阳性,该患者临床诊断肺部感染合并陈旧性肺结核。结论采用磁纳米捕获技术富集痰液中的结核分枝杆菌,可显著提高PCR检测的灵敏度。

Mycobacterium tuberculosis in sputum detected with magnetic nano-particles and PCR

Objective To capture M.tuberculosis in the sputum using nanotechnology and to increase the sensitivity of PCR for rapid diagnosis of tuberculosis.Methods Conventional smears and PCR served as the control.M.tuberculosis in sputum samples from 187 patients with tuberculosis(TB) and non-tuberculous respiratory diseases was captured and enriched with magnetic nano-particles,lysed,and then amplified by PCR.Results Of 152 sputum samples from patients with TB,41(27.0%) were positive according to sputum smears for acid-fast bacilli,72(47.4%) were positive according to conventional PCR,and 126(82.9%) were positive according to nano-particle capture and PCR.Of 35 sputum samples from patients with non-tuberculous respiratory diseases,all were negative according to smears but 1 sample from a patient with a pulmonary infection and old TB was positive according to PCR and capture-PCR.Conclusion M.tuberculosis in the sputum can be captured and enriched with magnetic nano-particles to increase the sensitivity of PCR.