An effective inducer of dopaminergic neuron-like differentiation |
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Authors: | Wenyu Fu Cui Lv Wenxin Zhuang Dandan Chen E Lv Fengjie Li Xiaocui Wang |
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Institution: | 1 Department of Histology and Embryology, Weifang Medical University, Weifang 261053, Shandong Province, China 2 Stem Cell Research and Transplantation Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China |
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Abstract: | Rat bone marrow-derived mesenchymal stem cells were cultured and passaged in vitro. After induction with basic fibroblast growth factor for 24 hours, passage 3 bone marrow-derived mesenchymal stem cells were additionally induced into dopaminergic neurons using three different combinations with basic fibroblast growth factor as follows: 20% Xiangdan injection; all-trans retinoic acid + glial-derived neurotrophic factor; or sonic hedgehog + fibroblast growth factor 8. Results suggest that the bone marrow-derived mesenchymal stem cells showed typical neuronal morphological characteristics after induction. In particular, after treatment with sonic hedgehog + fibroblast growth factor 8, the expressions of nestin, neuron-specific enolase, microtubuleassociated protein 2, tyrosine hydroxylase and vesicular monoamine transporter-2 in cells were significantly increased. Moreover, the levels of catecholamines in the culture supernatant were significantly increased. These findings indicate that Xiangdan injection, all-trans retinoic acid + glial-derived neurotrophic factor, and sonic hedgehog + fibroblast growth factor 8 can all induce dopaminergic neuronal differentiation from bone marrow-derived mesenchymal stem cells. In particular, the efficiency of sonic hedgehog + fibroblast growth factor 8 was highest. |
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Keywords: | neural regeneration stem cells mesenchymal stem cells dopaminergic neuron induction differentiation Xiangdan injection all-trans retinoic acid sonic hedgehog fibroblast growth factor 8 catecholamine grants-supported paper photographs-containing paper neuroregeneration |
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