| 一株鸭乙型肝炎病毒DHBV全基因的克隆和序列分析 |
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| 引用本文： | 梁蔚芳,刘志华,何海棠,骆抗先. 一株鸭乙型肝炎病毒DHBV全基因的克隆和序列分析[J]. 南方医科大学学报, 2004, 24(4): 404-407 |
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| 作者姓名： | 梁蔚芳 刘志华 何海棠 骆抗先 |
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| 作者单位： | 第一军医大学南方医院感染内科, 广东, 广州, 510515 |
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| 基金项目： | 军队“十五”重点课题(01Z046)~~ |
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| 摘 要： | 目的 从本地樱桃谷鸭血清中克隆鸭乙型肝炎病毒(DHBV)DNA,并进行序列分析。方法 用PCR扩增DHBV全基因,连接至T载体上,挑选克隆进行测序分析,与GenBank中16株DHBV全基因组进行同源性比较,并进行分子进化树分析。结果 24-18与16株DHBV基因组比较,核苷酸同源性介于89.4%~99.3%之间,各开放阅读框氨基酸同源性比较显示差异显著地方位于P区。结论 24-18属于DHBV西方基因型中的一个亚型。
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| 关 键 词： | 鸭乙型肝炎病毒 同源性分析 基因型 |
| 文章编号： | 1000-2588(2004)04-0404-04 |
| 修稿时间： | 2003-10-26 |
Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus |
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| LIANG Wei-fang,LIU Zhi-hua,HE Hai-tang,LUO Kang-xian. Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus[J]. Journal of Southern Medical University, 2004, 24(4): 404-407 |
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| Authors: | LIANG Wei-fang LIU Zhi-hua HE Hai-tang LUO Kang-xian |
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| Affiliation: | LIANG Wei-fang,LIU Zhi-hua,HE Hai-tang,LUO Kang-xian Department of Infectious Diseases,Nangfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area. Methods The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses. Result Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions. Conclusion The isolated DHBV strain might belong to a subtype of the virus found in the western countries. |
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| Keywords: | duck hepatitis B virus homology analysis genetype |
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