血管紧张素-Ⅰ转换酶基因多态性与糖尿病及糖尿病肾病的关系

目的　探讨血管紧张素Ⅰ转换酶基因（ＡＣＥ基因） 多态性与糖尿病及糖尿病肾病（ＤＮ） 的易感性之间的关系。方法　应用聚合酶链式反应（ＰＣＲ）方法扩增４８ 例正常人、７４ 例胰岛素依赖型糖尿病（ＩＤＤＭ） 患者（ 其中４０ 例不伴ＤＮ，３４ 例合并ＤＮ） 、１０２ 例非胰岛素依赖型糖尿病（ＮＩＤＤＭ） 患者（５７ 例不伴ＤＮ，４５ 例伴ＤＮ） 的ＡＣＥ基因上２８７ｂｐ 片断，根据插入（Ｉ） 或缺失（Ｄ） 来判断其多态性。结果　健康对照组与ＩＤＤＭ 及ＮＩＤＤＭ 组ＡＣＥ 等位基因、基因型均无显著性差异（ Ｐ＞０－０５）；在ＩＤＤＭ 组中，Ｄ等位基因及ＤＤ基因型在伴ＤＮ亚组中显著升高；在ＮＩＤＤＭ 组中，与不伴ＤＮ者比较，伴ＤＮ者其Ｉ等位基因、ＩＩ基因型频率明显为低。结论ＡＣＥ 基因多态性与糖尿病易感性无关，与ＤＮ密切相关。

The relationship between angiotensin I converting enzyme gene polymorphism and diabetes mellitus and diabetic nephropathy

Objective To investigate whether ACE gene I/D polymorphism attributes to the susceptibility to diabetes mellitus and diabetic nephropathy (DN).Methods ACE I/D polymorphism was detected by polymerase chain reaction (PCR) amplification of DNA fragment.Forty eight control subjects , 40 normoalbuminuric IDDM patients,34 IDDM patients with DN,57 normoalbuminuric NIDDM and 45 NIDDM with DN were involved in this study. Amplification produced about 490bp and about 190bp products corresponding to the I and D alleles,respectively.Results Distribution of genotypes and alleles were not significantly different between control subjects and IDDM and NIDDM patients. The frequency of D allele and genotype DD was significantly higher in IDDM with DN than that without DN. The frequency of I allele and genotype II was significantly lower in NIDDM with DN . Conclusion DNA sequence differences in ACE gene may contribute to genetic susceptibility to DN in IDDM and NIDDM , but may not contribute to genetic susceptibility to diabetes mellitus.