D-半乳糖诱导小鼠肝损伤模型的方法研究 |
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引用本文: | 顾呈华,朱宝立,徐军,刘协,杨明晶. D-半乳糖诱导小鼠肝损伤模型的方法研究[J]. 职业与健康, 2009, 25(22): 2364-2366 |
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作者姓名: | 顾呈华 朱宝立 徐军 刘协 杨明晶 |
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作者单位: | 1. 南京医科大学公共卫生学院,江苏省南京市,210009 2. 江苏省疾病预防控制中心 |
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摘 要: | 目的探讨不同实验方法条件下,D-半乳糖所致小鼠肝损伤各项相关指标的变化及意义。方法采用4周腹腔注射染毒、急性腹腔注射染毒、急性尾静脉注射染毒的方法,测定不同剂量组小鼠血清中AIJT、AST及肝匀浆中SOD、GSH、MDA,肝细胞活性氧(Ros)水平、细胞凋亡率。结果4周腹腔注射染毒各组各指标与对照相比,差异无统计学意义(P〉n05),模型不成立。急性腹腔注射、急性尾静脉注射各剂量组相关指标变化提示,2种方法均可建立肝损伤模型,但尾静脉注射各组相关指标剂量-效应关系好于腹腔注射各组,灵敏度也更高。结论与其他2种方法相比,急性尾静脉注射D-半乳糖诱导小鼠肝损伤模型,具有染毒特异性高、剂量-效应关系好、指标特异性和灵敏度高等特点,是较理想的肝损伤模型复制方法。
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关 键 词: | D-半乳糖 肝损伤 活性氧 细胞凋亡 |
Study on the Methodology of D-galactose Induced Mouse Liver Injury Model |
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Affiliation: | GU Cheng-hua, ZHU Bao-li, XU Jun, et al. (Nanjing Medical University School of Public Health, Jiangsu, 210009, China} |
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Abstract: | [ Objective] To investigate the changes of the relevant indicators in the liver injury in mice induced by D-galactose under various experimental conditions. [ Methods ] D-galactose was delivered into mice by intraperitoneal injection in 4 weeks, acute intrap- eritoneal injection, and acute intravenous injection in tail, respectively. The ALT and AST activities in mouse serum, the SOD, GSH and MDA in liver homogenate and the reactive oxygen species I ROS } level and apoptosis percentages of hepatocytes were meas- ured respectively to evaluate the liver injury. [ Results] Comparing the group with intraperitoneal injection with control group, the re- lated liver indexes did not show significant differences { P 〉 0.05 ) , indicating that the liver injury model was unsubstantiated. Either acute intraperitoneal or intravenous injection of D-galactose could form liver injury model, revealed by the above mentioned indexes. Intravenous injection was prior to intraperitoneal injection in establishing the mouse liver injury model, with better dose-response re- lationship and higher sensitivity. [ Conclusion] In contrast to the other 2 experimental methods, intravenous injection of D-galactose is more effective in inducing mouse liver injury with better specificity, sensitivity and dose-response relationship, and is suggested a potent method in coping model of liver injuries. |
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Keywords: | D-galactose Liver injury Reactive oxygen species ( ROS ) Apoptosis |
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