δ阿片受体激动剂D-丙2,D-亮5脑啡肽对脓毒症大鼠死亡率的影响

目的观察δ阿片受体激动剂D-丙2,D-亮5脑啡肽（[D-Ala2,D-Leu5]enkephalin,DADLE）对脓毒症大鼠存活时间和死亡率的影响。方法采用盲肠结扎穿孔（CLP）方法制备大鼠脓毒症模型。120只SD大鼠（雌雄不拘）,按随机数字表法分为假手术组（n=20）、模型对照组（n=20）,及4个DADLE治疗组。各DADLE组按给药剂量及方式分为制模前给药3mg/kg组（n=20）、制模前给药5mg/kg组（n=20）、制模后给药3mg/kg组（n=20）、制模后给药5mg/kg组（n=20）。观察各组大鼠CLP术后的存活时间,计算7d累积死亡率。结果与CLP模型未治疗组比较,各DADLE治疗组大鼠CLP术后的存活时间显著延长（P〈0.05）,各组7d死亡率分别为85%、60%、70%及75%,均较CLP组明显降低 但各DADLE治疗组之间无统计学差异（P〉0.05）。结论δ阿片受体激动剂DADLE能够明显延长脓毒症大鼠的存活时间,降低脓毒症大鼠的死亡率。

Effect of delta-opioid receptor agonist on mortality in rats with sepsis

Objective To investigate the effects of delta-opioid receptor agonist [D-Ala2,D-Leu5] enkephalin （DADLE） injection on the survival time and mortality in rats with sepsis. Methods Sepsis model of rats was replicated by cecal ligation and puncture （CLP）. 120 SD rats were randomly divided into 6 groups （20 in each）：sham operation group,CLP model group,and 4 DADLE treatment groups,including DADLE1 group （treated before CLP,3 mg/ kg）,DADLE2 group （treated before CLP,5 mg/kg）,DADLE3 group （treated after CLP,3 mg/kg） and DADLE4 group （treated after CLP,5 mg/kg）. The survival time after CLP and the 7 d cumulative mortality were recorded. Results The 7 d cumulative mortality of each DADLE group was 85%,60%,70% and 75%,respectively. Compared with the CLP model group,treatment with DADLE could significantly prolong the survival time （P0.05） and decline the mortality. However,there were no statistical differences among the four DADLE groups （P0.05）. Conclusion Delta-opioid receptor agonist DADLE can obviously prolong the survival time and decline the mortality in septic rats.