| 电针对局灶性脑缺血大鼠海马神经细胞凋亡及Bcl-2 Bax caspase-3表达的影响 |
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| 引用本文： | 周红艳,范军铭,王军.电针对局灶性脑缺血大鼠海马神经细胞凋亡及Bcl-2 Bax caspase-3表达的影响[J].辽宁中医杂志,2009(8):1427-1429. |
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| 作者姓名： | 周红艳 范军铭 王军 |
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| 作者单位： | 河南省中医药研究院药理实验室; |
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| 基金项目： | 国家自然科学基金资助项目(39270837);;河南省自然科学基金资助项目(984021000) |
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| 摘 要： | 目的:研究电针对局灶性脑缺血再灌注大鼠神经细胞凋亡和相关基因表达的影响。方法:SD雄性大鼠随机分为假手术组、模型对照组、尼莫地平组、电针治疗组(左侧肩禺、外关、髀关、足三里)、非穴位对照组(左侧天泉与曲泽连线中点、曲泽与郗门中点、五里与阴胞连线中点和膝关与中都连线中点)。采用线拴法造成大鼠局灶性脑缺血再灌注模型(MCAO),分别于缺血后即刻、再灌注后即刻和再灌注后2h电针,共3次。末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记(TUNEL)法测定海马凋亡神经细胞,免疫组织化学方法研究海马神经细胞caspase-3、Bcl-2和Bax基因表达。结果:与假手术组比较,MCAO大鼠海马凋亡细胞明显增加,caspase-3和Bax基因表达增强,Bcl-2/Bax比值显著降低;电针能明显降低MCAO大鼠海马TUNEL、caspase、Bax和Bcl-2阳性细胞数,Bcl-2/Bax比值显著升高。结论:电针可通过抑制神经细胞凋亡途径实现对脑缺血及缺血再灌注损伤的保护作用。
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| 关 键 词： | 电针 脑缺血再灌注 细胞凋亡 caspase-3 Bcl-2 Bax |
Effects of Electroacupuncture on Neuron Apoptosis and Related Gene Expression in Hippocampus of Rats with Focal Cerebral Ischemia-reperfusion Injury |
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| ZHOU Hong-yan,FAN Jun-ming,WANG Jun.Effects of Electroacupuncture on Neuron Apoptosis and Related Gene Expression in Hippocampus of Rats with Focal Cerebral Ischemia-reperfusion Injury[J].Liaoning Journal of Traditional Chinese Medicine,2009(8):1427-1429. |
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| Authors: | ZHOU Hong-yan FAN Jun-ming WANG Jun |
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| Institution: | Henan Academy of Chinese Medicine;Zhengzhou 450004;Henan;China |
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| Abstract: | Objective:To observe the effect of electroacupuncture(EA)on neuron apoptosis in hippocampus of rats with cerebral ischemia and reperfusion injury.Methods:Male SD rats were randomly divided into sham-operation group,model contral group,nimodipine group,EA group and non-acupoint contral group.The model of focal cerebral ischemia-reperfusion(MCAO) in rat was established by Longa's thread method.EA(10Hz,1.5-3V,duration of 0.6ms) was applied to left"Jianyu"(LI15),"Waiguan"(TE5),etc.for EA group,and the mid-point... |
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| Keywords: | Electroacupuncture Focal cerebral ischemia/reperfusion Neuron apoptosis caspase-3 Bcl-2 Bax |
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