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| 全脑缺血-再灌注对海马CA1区GAD65表达的影响及意义 | |
| 引用本文： | 许林峰,马文裴,刘静,冯忠堂. 全脑缺血-再灌注对海马CA1区GAD65表达的影响及意义[J]. 中国实用神经疾病杂志, 2008, 11(6): 38-39 |
| 作者姓名： | 许林峰 马文裴 刘静 冯忠堂 |
| 作者单位： | 1. 昆明医学院,神经科学研究所,昆明,650031;昆明医学院,第二附属医院神经外科,昆明,650031 2. 昆明医学院,生理教研室,昆明,650031 3. 昆明医学院,第二附属医院神经外科,昆明,650031 4. 昆明医学院,神经科学研究所,昆明,650031 |
| 摘 要： | 目的探讨全脑缺血-再灌注对成年大鼠海马CA1区GAD65表达的影响及意义。方法成年雄性SD大鼠24只,随机分为3组:假手术组(SH)、缺血-再灌注3d组(IR-3)及缺血-再灌注7d组(IR-7),每组8只。采用四动脉阻断法制作全脑缺血-再灌注模型,应用免疫组织化学方法检测海马CA1区谷氨酸脱羧酶(glutamic acid decarboxylase,GAD)同工酶GAD65的表达变化。结果与假手术组相比,IR-3组GAD65的表达明显增多,IR-7组恢复正常。结论GABA能中间神经元对缺血相对耐受;全脑缺血-再灌注3dGAD65的表达增多可能是一种代偿性的机制,以减轻脑缺血后的高兴奋性。 |
| 关 键 词： | 脑缺血 再灌注 谷氨酸脱羧酶 海马 大鼠 |
| 文章编号： | 1673-5110(2008)06-0038-02 |
| 修稿时间： | 2008-04-10 |
Effect and significance of global ischemia-reperfusion on the expression of GAD65 in hippocampal CA1 region |
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| Xu Linfeng,),Ma Wenpei),Liu Jing),et al.. Effect and significance of global ischemia-reperfusion on the expression of GAD65 in hippocampal CA1 region[J]. Chinese Journal of Practical Neruous Diseases, 2008, 11(6): 38-39 | |
| Authors: | Xu Linfeng ) Ma Wenpei) Liu Jing) et al. |
| Affiliation: | Xu Linfeng1,2),Ma Wenpei3),Liu Jing2),et al. 1) Institute for Research on Neuroscience 2)Department of Neurosurgery,Second Affiliated Hospital 3) Department of Physiology,Kunming Medical College,Kunming 650031,China |
| Abstract: | Objective To observe the effect of global ischemia-reperfusion on the expression of GAD65 in the hippocampal CAl region of adult rats.Methods 24 male SD rats were randomly divided into three groups:sham-operation group(SH,n=8),ischemia-reperfusion three days(IR-3,n=8) and seven days group(IR-7,n=8).Global ischemic episode was achieved by 4-vessel occlusion.Immunohistochemical method was applied to observe the expression of GAD65 in hippocampal CAl region.Results At ischemia-reperfusion third day,the immunoreactivities of the GAD 65 were markedly elevated in the CA1 region compared with sham operated group,but the immunoreactivities recovered to the sham level at ischemia-reperfusion seventh day.Conclusion GABAergic interneurons relatively resistant to ischemia-reperfusion injury,and the increasing expression of GAD65 3d after ischemia-reperfusion can be a compensatory mechanism to reduce the hyperexcitability associated with ischemia-reperfusion. |
| Keywords: | Brain ischemia Reperfusion GAD65 Hippocampus Rat |
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