喉癌EGFR和PCNA表达及DNA含量的研究

研究喉鳞癌中表皮生长因子受体、增殖细胞核抗原的表达和ＤＮＡ含量，分析各指标间的相关性及与喉癌临床生物学行为和预后的关系。方法：用免疫组化ＳＰ法和图像分析仪检测正常喉粘膜、喉鳞癌的ＥＧＦＲ、ＰＣＮＡ和ＤＮＡ指数，结合临床资料进行分析。结果：喉鳞癌的ＥＧＦＲ的阳性表达率为５４．８％，与正常喉组织的差异有统计学意义，但ＥＧＦＲ的阳性表达与喉癌的病理分级和５年生丰率无显著相关，而ＰＣＮＡ阳性表达和ＤＩ则随

Expression of EGFR and PCNA, and DNA content in squamous cell carcinoma of larynx

OBJECTIVE: To investigate the expression of epidermal growth factor (EGF), proliferating cell nuclear antigen (PCNA) and DNA index (DI) in laryngeal carcinoma, to analyse the correlation between these index and the biological characteristics of laryngeal carcinoma and their values of clinical prognosis. METHOD: Immunohistochemical staining was used to detect the expression of EGFR and PCNA in laryngeal cancer and normal tissue, and with MIPS-I image analysis system DNA contents of cancer cell were measured and made out DNA index. RESULT: The positive rate of EGFR in laryngeal carcinoma was 54.8%, and it was negative in all 10 normal laryngeal mucosa specimens (P < 0.01). The expression of EGFR did not correlate with histological grading and 5-years survival rate (P > 0.05), The positive expression of PCNA and DNA contents in the laryngeal carcinoma were increased with the decrease of tumorous differentiation (P < 0.05). With the increasing of PCNA positive expression and DI, the prognosis of the patients were poorer (P < 0.05). CONCLUSION: EGFR may be related to the process of carcinogenesis in laryngeal carcinoma and was used as an early biomarker identifying premalignant lesions which had the greatest risk of carcinogenesis. PCNA and DI were simultaneously detected can be used as the prediction of tumor malignancy and prognosis.