| 齐墩果酸对白血病HL-60细胞PI3K、Akt表达的影响 |
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| 引用本文： | 王迪迪,朱贵明,张鹏霞. 齐墩果酸对白血病HL-60细胞PI3K、Akt表达的影响[J]. 肿瘤药学, 2013, 0(4): 268-270,281 |
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| 作者姓名： | 王迪迪 朱贵明 张鹏霞 |
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| 作者单位： | 佳木斯大学基础医学院生物化学教研室,黑龙江佳木斯154007 |
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| 基金项目： | 国家自然科学基金资助项目（81070428）;黑龙江省卫生厅科研课题（2011-438）;佳木斯大学科研项目（s2010-102）. |
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| 摘 要： | 目的研究齐墩果酸(Oleanic acid,OA)对人白血病HL-60细胞PI3K、Akt表达的影响。方法 OA作用HL-60细胞后,采用透射电镜观察细胞形态,RT-PCR法检测HL-60细胞PI3K、Akt的mRNA表达。结果 OA作用于HL-60细胞后,细胞形态改变明显,出现凋亡小体,且PI3K、Akt的mRNA表达下调(P<0.01)。结论 OA可抑制HL-60细胞增殖,这可能与其下调HL-60细胞中PI3K、Akt的表达有关。
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| 关 键 词： | 齐墩果酸 HL-60 PI3K Akt |
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| Wang Didi,Zhu Guiming,Zhang Pengxia. ------------[J]. Anti-Tumor Pharmacy, 2013, 0(4): 268-270,281 |
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| Authors: | Wang Didi Zhu Guiming Zhang Pengxia |
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| Affiliation: | (Biochemistry Department, Basic Medical College of Jiamusi University, Jiamusi, Heilongjiang, 154007, China) |
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| Abstract: | Objective To study the Effect of oleanolic acid （OA） on the expression of Phosphoinositide 3-kinase （PI3K） and Akt genes in leukaem HL-60 cells. Methods Transmission electro microscopy was applied to observe the morphological and structural changes of the HL-60 cells after being treated by OA, and the mRNA expressions of PI3K and Akt were detected by P,.T- PCR. Results After being treated by OA, the HL-60 cells had obvious morphological and structural changes under the transmission electro microscopy, and apoptosis body was observed as well. The mRNA expression of PI3K and Akt were down regulated （P〈 0.01）. Conclusions OA could inhibit the proliferation of ilL-60 cells by down-regulating the expression of P13K and Akt. |
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| Keywords: | Leukaemia cancer Oleanolic acid P13K Akt HL-60 cell |
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