高效毛细管电泳法测定黄柏中盐酸小檗碱和盐酸巴马汀

 目的建立同时测定黄柏药材中盐酸小檗碱和盐酸巴马汀含量的方法。方法采用高效毛细管电泳法。运行缓冲液为50mmol·L^-1Na₂B₄O₇-CH₃OH(85:15),pH7.0;操作电压14kV;检测波长265nm。结果在选定的电泳条件下,盐酸小檗碱和盐酸巴马汀的线性范围分别为0.005～0.08和0.002～0.03g·L^-1,r=0.9997和0.9985;方法的加样回收率分别为98.3%和99.4%,精密度分别为1.89%和2.21%。结论所建立的分析方法具有准确、简便、快速的特点,可以用于黄柏药材中盐酸小檗碱和盐酸巴马汀的同时分析检测。

Simultaneous determination of berberine hydrochloride and palmatine hydrochloride in Cortex Phellodendri chinensis by capillary electrophoresis

OBJECTIVE To establish an analytical method for simultaneous determination of berberine hydrochloride and palmatine hy-drochloride in Cortex Phellodendri chinensis.METHODS High performance capillary electrophoresis was used with the mobile phase consisting of 50 mmol·L^-1 sodium tetraborate solution-methanol (85:15, pH 7.0). The applied voltage was 14 kV and detection wavelength was at 265 nm. RESULTS The linear curves for berberine hydrochloride and palmatine hydrochloride were obtained in the range of 0.005～0.08 mg·mL^-1(r=0.999 7) and 0.002～0.03 g·L-1(r=0.998 5),respectively.The recovery was 98.3% with RSD of 1.89% for berberine hydrochloride and 99.4% with RSD of 2.21% for palmatine hydrochloride. CONCLUSION The method can be applied to the simultaneous analysis of berberine hydrochloride and palmatine hydrochloride in Cortex Phellodendri chinensis.