| Transfection of p27^kip1 enhances radiosensitivity induced by ^60Co γ-irradiation in hepatocellular carcinoma HepG2 cell line |
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| 引用本文： | Guan XX,Chen LB,Ding GX,De W,Zhang AH. Transfection of p27^kip1 enhances radiosensitivity induced by ^60Co γ-irradiation in hepatocellular carcinoma HepG2 cell line[J]. World journal of gastroenterology : WJG, 2004, 10(21): 3103-3106. DOI: 10.3748/wjg.v10.i21.3103 |
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| 作者姓名： | Guan XX Chen LB Ding GX De W Zhang AH |
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| 基金项目： | the,National,Postdoctor,Research,Foundation,of,China(项目编号：2003034383) |
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| 摘 要： | AIM: To study the cell cycle alterations of human hepatoma cell line HepG2 in vitro after ^60Co γ-irradiation and further to examine the mechanisms underlying the enhancement of radiosensitivity to γ-irradiation in HepG2 transiently transfected with wild type p27^kip1. METHODS: The proliferation of HepG2 cells was evaluated with MTT assay, and the cell cycle profile and apoptosis were assessed by cell morphology, DNA fragmentation analysis and flow cytometry. HepG2 cells were transfectedwith p27^kip1 wild type by using Lipofectamine (LF2000), and the expression and subcellular localization of p27^kip1 in HepG2 were detected by immunocytochemistry.RESULTS: ^60Co γ-irradiation inhibited the growth of HepG2 cells in a dose-dependent manner. Apoptosis of HepG2 cells was induced 48 h after ~, ray exposure. Furthermore research was carried out to induce exogenous expression of p27^kip1 in HepG2. The expression of p27^kip1 induced G0/G1 phase arrest in HepG2 cells. The overexpression of p27^kip1 enhanced ^60Co γ-irradiation-induced radiosensitivity in HepG2 cells. CONCLUSION: Overexpression of p27^kip1 is a rational approach to improve conventional radiotherapy outcomes, which may be a possible strategy for human hepatoma therapy.
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| 关 键 词： | 基因转染 p27^kip1 辐射敏感度 ^60Co γ-照射 HepG2细胞系统 肝细胞癌 肿瘤 消化系统 |
| 收稿时间： | 2004-03-27 |
Transfection of p27kip1 enhances radiosensitivity induced by 60Co gamma-irradiation in hepatocellular carcinoma HepG2 cell line |
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| Guan Xiao-Xiang,Chen Long-Bang,Ding Gui-Xia,De Wei,Zhang Ai-Hua. Transfection of p27kip1 enhances radiosensitivity induced by 60Co gamma-irradiation in hepatocellular carcinoma HepG2 cell line[J]. World journal of gastroenterology : WJG, 2004, 10(21): 3103-3106. DOI: 10.3748/wjg.v10.i21.3103 |
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| Authors: | Guan Xiao-Xiang Chen Long-Bang Ding Gui-Xia De Wei Zhang Ai-Hua |
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| Affiliation: | Department of Oncology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu Province, China. |
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| Abstract: | AIM: To study the cell cycle alterations of human hepatoma cell line HepG(2) in vitro after (60)Co gamma-irradiation and further to examine the mechanisms underlying the enhancement of radiosensitivity to gamma-irradiation in HepG(2) transiently transfected with wild type p27(kip1). METHODS: The proliferation of HepG(2) cells was evaluated with MTT assay, and the cell cycle profile and apoptosis were assessed by cell morphology, DNA fragmentation analysis and flow cytometry. HepG(2) cells were transfected with p27(kip1) wild type by using Lipofectamine (LF2000), and the expression and subcellular localization of p27(kip1) in HepG(2) were detected by immunocytochemistry. RESULTS: (60)Co gamma-irradiation inhibited the growth of HepG(2) cells in a dose-dependent manner. Apoptosis of HepG(2) cells was induced 48 h after gamma ray exposure. Furthermore research was carried out to induce exogenous expression of p27(kip1) in HepG(2). The expression of p27(kip1) induced G(0)/G(1) phase arrest in HepG(2) cells. The overexpression of p27(kip1) enhanced (60)Co gamma-irradiation-induced radiosensitivity in HepG(2) cells. CONCLUSION: Overexpression of p27(kip1) is a rational approach to improve conventional radiotherapy outcomes, which may be a possible strategy for human hepatoma therapy. |
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