基于电子鼻及顶空-气质联用技术结合化学计量学区分不同产地的砂仁

目的:采用电子鼻及自动顶空-气相色谱-质谱联用(HS-GC-MS)技术从整体香气轮廓和具体香气成分两个方面检测不同产地砂仁的香气物质,研究砂仁气味电子鼻响应的物质基础。方法:以广东、云南、缅甸、越南和老挝等不同产地砂仁药材为研究对象,利用电子鼻和HS-GC-MS技术检测其挥发性成分,采用双标图分析样品-传感器-挥发性化合物三者的联系。结果:电子鼻的PCA双标图显示不同产地的砂仁能较好地被区分,识别指数为83.90%,其中国产的阳春砂仁归为一类,进口的砂仁为一类;采用HS-GC-MS共鉴别得到萜烯、酯、醇、酮及烷烃类等70种挥发性成分,各类成分及含量在不同产地的砂仁中有明显不同,分类与电子鼻的结果相一致;PLS双标图显示传感器s14和s8/s6/s3与萜烯类、酯类和醇类成分的高度相关性揭示了传感器与香气物质之间的内在关系。结论:利用化学计量学,电子鼻检测技术可区分不同产地的砂仁药材,结合自动顶空-气质联用色谱分析方法为砂仁的气味电子鼻响应的物质基础研究提供了进一步的实验依据。

Discrimination of Aromi Fructus from Different Origins Using Electronic Nose Combined with Headspace-Gas Chromatography-Mass Spectrometry Coupled and Chemometrics

Objective: To detect aroma characteristics of Aromi Fructus from different originsby using electronic nose combined with automatic headspace-gas chromatography-mass spectrometry (HS-GC-MS)in two aspects, namely overall aroma profile and specific aroma composition, in order to study material basis of Aromi Fructus''s odors. Method: With Aromi Fructus of different varieties and from different origins-Guangdong,Yunnan,Burma,Vietnam and Laos as a research subject,the volatile components were detected by using electronic nose and HS-GC-MS technique.A bioplot was used to analyze the correlation among samples,E-nose gas sensors and chemical volatile compounds. Result: E-nose showed a good differentiation of Aromi Fructus from different origins, with the identification index of 83.9%.Homebred Amomum villosum was classified in class A, and imported Aromi Fructus was classified in class A.A total of 70 volatile components were identified by HS-GC-MS, including terpenes,esters,alcohols,ketone and alkane.Aromi Fructus of different varieties and from different origins have different composition and content, with the same classification with the results of E-nose.A bioplot of PLS displayed the high correlation between sensors s14,s8/s6/s3 and terpenes,esters and alcohols indicating that special sensors were highly correlated with some aroma constituents. Conclusion: Advanced chemometrics and E-nose are used to discriminate Aromi Fructus from different origins.By combining with HS-GC-MS technique, this study provides further experimental evidence for material bases of Aromi Fructus''s odors.