当归挥发油对人脐静脉内皮细胞增殖作用的谱效关系

目的:探讨不同产地当归挥发油与人脐静脉内皮细胞增殖作用的相关关系。方法:利用水蒸气蒸馏法提取当归挥发油,采用GC-MS联用分析技术建立44批不同产地当归药材挥发油指纹图谱,质谱检索库及保留指数法鉴定各色谱峰所示化学成分,用噻唑蓝染色(MTT)实验考察不同产地当归挥发油对HUVECs细胞的增值率,再通过线性回归分析方法,建立并分析当归挥发油促HUVECs细胞增殖作用的相关关系。结果:通过线性回归分析得色谱峰X_1,X_2与细胞增殖率呈负相关的关系,其余色谱峰与细胞增殖率均呈正相关的关系,且其中X_3,X_6,X_7号色谱峰回归系数较大,是当归挥发油促HUVECs细胞增殖的主要化学成分。结论:该方法能快速、有效地建立当归挥发油谱效相关关系,可为当归挥发油化学成分药理性质的研究提供参考依据。

Correlation Analysis Between Volatile Oil from Angelicae Sinensis Radix and Its Effect on HUVECs Proliferation

Objective: To study the correlation between the GC-MS fingerprints of volatile oil from Angelicae Sinensis Radix of different regions and its effect on proliferation. Method: The volatile oil from Angelicae Sinensis Radix was extracted by steam distillation, and GC-MS analysis technology was used to establish the fingerprints of volatile oil from 44 batches of Angelicae Sinensis Radix of different regions. Mass spectrometry database and retention index were used to identify the chemical components indicated in various chromatographic peaks, and then Methyl thiazolyltetazolium(MTT) was used to investigate the effect of volatile oil from Angelicae Sinensis Radix of different regions on HUVECs proliferation. Finally, the fingerprint-effect relationship of volatile oil and promoting HUVECs proliferation effect could be established and analyzed by linear regression analysis. Result: Linear regression analysis showed that, X₁ and X₂ peaks were negatively correlated with cell proliferation rate, and other peaks showed positive correlation. Among them, X₃, X₆ and X₇ peaks had larger linear regression coefficient, showing main constituents for promoting HUVECs proliferation. Conclusion: This method can quickly and effectively establish the spectrum-effect relationship for volatile oil from Angelicae Sinensis Radix, providing experimental basis for studying their pharmacological properties.