| 汉坦病毒诱导人脐静脉内皮细胞HSP70的表达及意义 |
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| 引用本文： | 余璐,马恒,刘彦仿,杨守京. 汉坦病毒诱导人脐静脉内皮细胞HSP70的表达及意义[J]. 细胞与分子免疫学杂志, 2005, 21(1): 9-12 |
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| 作者姓名： | 余璐 马恒 刘彦仿 杨守京 |
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| 作者单位： | 1. 第四军医大学基础部病理学教研室,陕西,西安,710032
2. 第四军医大学基础部生理学教研室,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目(No. 30271186) |
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| 摘 要： | 目的: 研究人脐静脉内皮细胞 (HUVEC)感染汉坦病毒(Hantavirus, HTV)后应激反应的规律及意义。方法: 采用免疫细胞化学染色法和核酸分子原位杂交, 检测热休克蛋白 70(HSP70)的表达; 用RT --PCR观察HSP70mRNA水平变化的规律。结果: HTV感染HUVEC后, 免疫细胞化学染色法可检出HSP70呈高表达, 原位杂交发现细胞质内有HSP7mRNA的阳性信号。感染后不同时间点RT- PCR的结果表明与对照组相比较, HSP70mRNA的水平在感染后迅速升高并持续高表达 (P<0. 05 )。结论: HTV可诱导HUVEC高表达HSP70。HSP70可能具有抑制病毒复制和保护内皮细胞的作用。
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| 关 键 词： | 汉坦病毒 人脐静脉内皮细胞 热休克蛋白70 |
| 文章编号： | 1007-8738(2005)01-0009-04 |
Expression of HSP70 in human umbilical vein endothelial cells induced by Hantavirus |
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| YU Lu,Ma Heng,LIU Yan-fang,YANG Shou-jing. Expression of HSP70 in human umbilical vein endothelial cells induced by Hantavirus[J]. Chinese journal of cellular and molecular immunology, 2005, 21(1): 9-12 |
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| Authors: | YU Lu Ma Heng LIU Yan-fang YANG Shou-jing |
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| Affiliation: | Department of Pathology ,Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | AIM: To study stress response in human umbilical vein endothelial cells(HUVEC) infected with Hantavirus (HTV). METHODS: The expression of HSP70 was detected by immunocytochemical staining, in situ nucleic acid hybridization and RT-PCR. RESULTS: After being infected with HTV, the expression of HSP70 in HUVEC increased. RT-PCR showed that the level of HSP70 mRNA increased rapidly and kept at a high level in comparison with the normal control(P<0.05). CONCLUSION: The over-expression of HSP70 in HUVECs can be induced by Hantavirus, which may play a role in the inhibition of virus reproduction and the protection of cells from virus infection. |
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| Keywords: | Hantavirus human umbilical vein vascular endothelial cell heat shock protein 70 |
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