壳寡糖促神经元生长的作用

目的研究壳寡糖（COSs）对大鼠神经元生长的影响。方法以体外培养的背根神经节（DRG）和DRG神经元为研究模型，通过神经丝蛋白-H（NF—H）免疫荧光细胞化学染色和LeicaQWin软件检测不同浓度壳寡糖（0．0、0．1、0．2mg／m1）作用5d对DRG和DRG神经元突起生长的影响；通过Westernblot检测不同浓度壳寡糖（0．05、0．1、0．2mg／m1）作用DRG神经元12h后，对DRG神经元中NF—H和生长相关蛋白-43（GAP-43）表达的影响。结果与对照组相比，中、高剂量壳寡糖（0．1、0．2mg／m1）显著促进DRG突起的生长（均P〈0．01）；0．2mg／ml剂量的壳寡糖明显促进DRG神经元突起的生长（P〈0．05）。与对照组相比，体外培养的DRG神经元加入壳寡糖作用12h后，0．2mg／ml剂量壳寡糖组的NF—H和GAP43表达量明显增加（分别P〈0．05和〈0．01）。结论壳寡糖可有促进体外培养的DRG和DRG神经元突起的生长，并可促进NF—H、GAP43的表达。

Effect of chitooligosaccharides on the neurite outgrowth of neurons

Objective To determine the effect of chitooligosaccharides （COSs） on the neurite outgrowth neuron of rats in vitro. Methods Dorsal root ganglion （DRG） explant and dissociated DRG neuron were cul- tured, and neurofilament-H （NF-H） fluorescent immunocytochemistry and Leica QWin software was performed to detect the neurite outgrowth of cultured DRG and DRG neuron treated with COSs （0.05, 0.1 and 0.2 mg/ ml）. Western blot was used to analyze the expression changes of NF-H and growth associated protein-43 （GAP-43） 12 h after treatment with COSs （0.05, 0. 1 and 0.2 mg/ml） in the dissociated DRG neuron. Re- suits The result of NF-H fluorescent immunocytochemistry showed that compared with the control group COSs （0. 1 mg/ral and 0, 2 mg/ml） promoted the neurite outgrowth of cultured DRG （P 〈0.01）. The effect of dif- ferent concentrations of COSs on neurite outgrowth of DRG neuron was similar to the effect on DRG. Compared with the control group, 0.2 mg/ml COSs promoted the neurite outgrowth of cultured DRG neuron （P 〈0.05）. The results of Western blot showed that compared with control group, the protein levels of NF-H and GAP43 were up-regulated in disscciated DRG neurons after treated with O. 2 mg/ml COSs treatment for 12 h （P 〈0.05 and P 〈0.01 ）. Conclusion COSs could promote the neurite outgrowth of cultured DRG and DRG neuron, and increase the expression of NF-H and GAP43.