| UPLC测定茜草炭中4种醌类成分的含量 |
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| 引用本文： | 陈星,王侃,单鸣秋,丁安伟.UPLC测定茜草炭中4种醌类成分的含量[J].中国中药杂志,2012,37(19):2922-2925. |
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| 作者姓名： | 陈星 王侃 单鸣秋 丁安伟 |
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| 作者单位： | 南京中医药大学 江苏省方剂高技术研究重点实验室,江苏 南京,210046 |
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| 基金项目： | 中医药行业科研专项项目资助(201107007) |
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| 摘 要： | 目的:建立同时测定茜草炭中异茜草素、羟基茜草素、1,3,6-三羟基-2-甲基蒽醌、大叶茜草素含量的超高效液相色谱法.方法:采用Acquity BEHC18色谱柱(2.1 mm×50 mm,1.7μm),流动相为甲醇-0.3％甲酸溶液,梯度洗脱,流速0.2mL·min-1,进样量2μL,柱温30 ℃,检测波长276 nm.结果:异茜草素、羟基茜草素、1,3,6-三羟基-2-甲基蒽醌、大叶茜草素的质量浓度与峰面积分别在0.69～34.44 mg·L-1(r=0.999 9),0.66～ 33.2 mg·L-1(r=0.999 7),0.68～34.08 mg·L-1(r=0.999 9),1.07～ 53.52 mg·L-1(r=0.9999)呈良好线性;平均回收率分别为96.95％,95.75％,102.5％,96.15％,RSD均小于3％.结论:该方法简便、快速、准确,可作为茜草炭质量控制的一个有效方法.
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| 关 键 词： | 茜草炭 异茜草素 羟基茜草素 1 3 6-三羟基-2-甲基蒽醌 大叶茜草素 UPLC |
| 收稿时间： | 2012/3/26 0:00:00 |
Simultaneous determination of four quinone compounds in carbonized Rubiae Radix et Rhizoma by UPLC |
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| CHEN Xing,WANG Kan,SHAN Ming-qiu and DING An-wei.Simultaneous determination of four quinone compounds in carbonized Rubiae Radix et Rhizoma by UPLC[J].China Journal of Chinese Materia Medica,2012,37(19):2922-2925. |
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| Authors: | CHEN Xing WANG Kan SHAN Ming-qiu and DING An-wei |
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| Institution: | Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China |
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| Abstract: | |
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| Keywords: | carbonized Rubiae Radix et Rhizoma purpuroxanthine purpurin 1 3 6-trihydroxy-2-methylanthraquinone rubimaillin UPLC |
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