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| 黄葵胶囊对阿霉素肾病肾组织炎症信号通路p38MAPK的干预作用 | |
| 引用本文： | 赵青,万毅刚,孙伟,王朝俊,魏晴雪,陈好利,孟宪杰. 黄葵胶囊对阿霉素肾病肾组织炎症信号通路p38MAPK的干预作用[J]. 中国中药杂志, 2012, 37(19): 2926-2934 |
| 作者姓名： | 赵青 万毅刚 孙伟 王朝俊 魏晴雪 陈好利 孟宪杰 |
| 作者单位： | 1. 南京中医药大学 中西医结合鼓楼临床医学院,江苏 南京,210029 2. 南京大学 医学院 附属鼓楼医院 中医科,江苏 南京,210008 3. 江苏省中医院 肾内科,江苏 南京,210029 4. 昆山市中医院,江苏 昆山,215300 |
| 基金项目： | 南京市卫生青年人才培养工程项目;江苏高校优势学科建设工程项目 |
| 摘 要： | 目的:阐明黄蜀葵花(Abelmoschus manihot,AM)提取物制剂——黄葵胶囊(Huangkui capsule,HKC)调节阿霉素肾病(adriamycin-induced nephropathy,ADRN)模型p38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路从而改善肾组织炎症性损伤的机制.方法:将19只SD大鼠随机分为假手术组、对照组和HKC组.对HKC组和对照组大鼠行右侧肾摘除术,并在4周内分2次经颈静脉注射阿霉素(adriamycin,ADR)建立ADRN模型.HKC组大鼠于第2次注射ADR后经灌胃给予HKC悬浊液,对照组和假手术组大鼠同时经灌胃给予蒸馏水,共干预4周.在HKC和蒸馏水干预前和干预后第1,2,3,4周末,分别称量大鼠体重,并检测24 h尿蛋白排泄量(24 h urinary protein excretion,Upro).肾摘除术后第8周末处死全部大鼠,抽取血液,摘除左肾并称重,观察血清生化指标、肾小球形态特征、肾小球α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、Ⅰ型胶原(collagen type Ⅰ)表达特征、肾小球巨噬细胞(macrophage,Mφ)浸润特征等,并且检测肾组织转化生长因子(transforming growth factor,TGF)-β1,p38MAPK,磷酸化p38MAPK(phosphorylated p38MAPK,p-p38MAPK)蛋白相对表达量.结果:与对照组大鼠比较,HKC改善了模型鼠的蛋白尿和血清白蛋白(albumin,Alb),抑制了模型鼠系膜细胞(mesangial cell,MC)增殖、细胞外基质(extracellular matrix,ECM)及其胶原成分沉积,减轻了肾小球α-SMA,Ⅰ型胶原表达,减轻了肾小球ED1+,ED3+浸润,下调了模型鼠肾组织TGF-β1,p-p38MAPK蛋白表达.结论:HKC在体内具有减轻肾组织炎症性损伤的作用;HKC通过下调肾组织p38MAPK信号通路中关键信号分子——p-p38MAPK的蛋白表达,干预其相关信号通路的信号转导途径,减少肾组织内TGF-β1的表达和炎症细胞的浸润、活化,从而改善肾组织的炎症性损伤. |
| 关 键 词： | 黄葵胶囊 阿霉素肾病 炎症信号通路 p38MAPK 磷酸化p38MAPK |
| 收稿时间： | 2012-05-02 |
Effects of Huangkui capsule on renal inflammatory injury by intervening p38MAPK signaling pathway in rats with adriamycin-induced nephropathy |
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| ZHAO Qing,WAN Yi-gang,SUN Wei,WANG Chao-jun,WEI Qing-xue,CHEN Hao-li and MENG Xian-jie. Effects of Huangkui capsule on renal inflammatory injury by intervening p38MAPK signaling pathway in rats with adriamycin-induced nephropathy[J]. China Journal of Chinese Materia Medica, 2012, 37(19): 2926-2934 | |
| Authors: | ZHAO Qing WAN Yi-gang SUN Wei WANG Chao-jun WEI Qing-xue CHEN Hao-li MENG Xian-jie |
| Affiliation: | Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjng 210008, China;Department of Traditional Chinese Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China;Department of Nephrology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, China;Kunshan Hospital of Traditional Chinese Medicine, Kunshan 215300, China;Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjng 210008, China;Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjng 210008, China;Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjng 210008, China |
| Abstract: | |
| Keywords: | Huangkui capsule(HKC) adriamycin-induced nephropathy inflammation signaling pathway p38 mitogen-activated protein kinase(MAPK) phosphorylated p38MAPK(p-p38MAPK) |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |