Calibration of photodiode measurements of cell motion by a transmission optical lever method |
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| Authors: | Brian A. Clark Richard Hallworth Burt N. Evans |
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| Affiliation: | (1) Auditory Physiology Laboratory (Audiology) and Department of Neurobiology and Physiology, Northwestern University, 60208 Evanston, IL, USA |
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| Abstract: | A method of calibrating photodiode measurements of cell motion is described. A transparent glass plane is inserted perpendicular to the light path, in front of the photodiode. When the glass plane is rotated, changing the angle between the plane and the light path, the image is displaced. The rotatable glass plane acts as an optical lever to simulate cell motion. The optical lever is easy to construct and offers excellent linearity and accuracy. |
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| Keywords: | Motion transducer Calibration Optical lever Photodiode Cell motility |
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