针刺“百会”“四神聪”对老年性痴呆大鼠认知行为及脑内超氧化物歧化酶的影响

目的:探讨采用针刺的方法对AD大鼠认知行为及脑内超氧化物歧化酶(SOD)活性的影响。方法:选用纯系健康雄性Wistar大鼠4 8只,随机分为假手术组、模型组、针刺组和西药组。采用β- 淀粉样蛋白向海马CA1区定向注射制作AD模型。采用针刺“百会”“四神聪”穴进行治疗。检测大鼠的穿梭箱实验成绩和脑组织内SOD的活性。结果:针刺模型大鼠的“百会”“四神聪”穴可以减少其在穿梭箱实验中的电击次数和电击时间,提高脑组织内SOD活性。结论:采用针刺的方法可明显改善AD大鼠的学习记忆能力,并能提高其脑内SOD的活性。

Effect of Acupuncture of "Baihui"(GV 20) and "Sishencong"(EX-HN 1) on Memory and Cerebral SOD Activity in Alzheimer's Disease Rats

Objective: To investigate the effect of acupuncture on the memory and SOD activity in the cerebral issues in Alzheimer's disease (AD) rats. Methods: 48 healthy Wister rats were randomly divided into control group (n=11), model group (n=10), medication group (n=10) and acupuncture group (n=11). AD model was established by microinjection of β-amyloid protein (β-AP)1-40 (1 μL) into the hippocampal CA1 region (AP: 2.22 mm; ML: 3.0 mm, DV: 2.8 mm). In acupuncture group, “Baihui”(GV 20) and “Sishencong”(EX-HN 1) were punctured with filiform needles which were lifted, thrust and twirled continuously for 5 min, and then retained for 20 min. The treatment was conducted once daily except Sundays and continuously for one month. Rats of medication group were fed with Piracetum (40 mg/mL, 6 mL/kg/time) once daily for 30 days. The rats' memory was detected with shuttle-box test and the activity of superoxide dismutase (SOD) in the cerebral issues assayed by enzymological method. Results: Shuttle box test showed that compared with control group, the times and duration of electric shock were significantly more and longer than those of model group (P<0.05); while compared with model group, the times and duration of electric shock of both acupuncture and medication groups were significantly more and longer (P<0.01). No significant differences were found between two groups in these two indexes, suggesting that both acupuncture and medication can improve AD rats' memory. In comparison with control group, SOD activity in the cerebral tissue lowered considerably; while in comparison with model group, SOD activity of both medication and acupuncture groups increased significantly (P<0.01), and no significant difference was found between the later two groups (P>0.05). Conclusion: Acupuncture treatment can improve AD rats' learning and memory and raise SOD activity in the cerebral tissue.