| 反相高效液相色谱法测定复方茵陈注射液中氯原酸和栀子甙的含量 |
| |
| 引用本文： | 韩晋 蔡光明. 反相高效液相色谱法测定复方茵陈注射液中氯原酸和栀子甙的含量[J]. 中国药学杂志, 1995, 30(3): 166-167 |
| |
| 作者姓名： | 韩晋 蔡光明 |
| |
| 作者单位： | 北京解放军３０２医院药剂科 |
| |
| 摘 要： | 采用反相高效液相色谱法测定复方茵陈注射液中主要成分氯原酸和栀子成的含量。氯原酸在３３５ｎｍ波长测定，栀子成在２３７ｎｍ波长测定。外标法定量，回收率分别为９３．６％和９７．５％。氯原酸和桅子戒回归方程分别为Ａ＝０．１８７＋０．８５８Ｃ（ｒ＝０．９９９３），Ａ＝０．２１＋６．０５５６Ｃ（ｒ＝０．９９８３）。方法操作简单，灵敏度及准确度均较高。
|
| 关 键 词： | 反相高效液相色谱法 氯原酸 栀子甙 |
| 收稿时间： | 1994-04-12; |
RP-HPLCmethod for the determination of chlorogenic acid andgardenoside in Artemisia capillaris compound injection |
| |
| Han Jing,Cai Guangming. RP-HPLCmethod for the determination of chlorogenic acid andgardenoside in Artemisia capillaris compound injection[J]. Chinese Pharmaceutical Journal, 1995, 30(3): 166-167 |
| |
| Authors: | Han Jing Cai Guangming |
| |
| Affiliation: | 302 Hospital,PLA,Beijing 100039 |
| |
| Abstract: | A RP-HPLC method for the determination of chlorogenic acid and gardenoside whichare the main components in Artemisia capillaris Thunb.Compound injection was established.A C18 col-wmn and a mobile phase of methanol-water(70:30) were employed.Chlorogenic acid was determinedat 335 nm and gardenoside was determined at 237 nm。 The recovery rate of the two components was93.6%and 97.5%, respectively。For chlorogenic acid,the regression equation was A=0.187 + 0。858C(r=0.9993) and for gardenoside is A=0.21+6.0556C(r=0.9983).The methOd is simple,sensitive and accurate。 |
| |
| Keywords: | RP-HPLC chlorogenic acid gardenoside |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
|
点击此处可从《中国药学杂志》下载免费的PDF全文 |
