| 水飞蓟宾对培养心肌细胞感染CoxSackie B5病毒的保护作用 |
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| 引用本文： | 章同华,沈茜.水飞蓟宾对培养心肌细胞感染CoxSackie B5病毒的保护作用[J].第二军医大学学报,1990,11(2):143-146. |
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| 作者姓名： | 章同华 沈茜 |
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| 作者单位： | 长海医院心血管内科
(章同华，王舒娅)，长海医院实验诊断科(沈茜) |
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| 摘 要： | 本文介绍用微孔培养板取代培养瓶培养新生大鼠心肌细胞,并用400 TCI_(50) 0.05 ml/孔CoxsackieB_5病毒感染心肌细胞.在感染后1,6、24及36h分别加入水飞蓟宾,观察到用水飞蓟宾后培养液中LDH、GOT均低于病毒对照组,感染后DNA合成率明显高于病毒对照组,以及96 h受感染细胞培养液中病毒滴度(Lg 3.95 TCID_(50))明显低于病毒对照组(Lg 6.95 TCID_(50)).本实验结果提示水飞蓟宾对Coxsackie B_5病毒感染培养的心肌细胞有明显保护作用,尤其是在感染后1～6 h给药,效果更好。
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| 关 键 词： | 心肌细胞培养 病毒感染 水飞蓟宾 |
Effect of Silybin on Coxsackie B_5 Virus Infected New Born Rat Heart Cells in Culture |
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| Zhang Tonghua,et al.Effect of Silybin on Coxsackie B_5 Virus Infected New Born Rat Heart Cells in Culture[J].Academic Journal of Second Military Medical University,1990,11(2):143-146. |
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| Authors: | Zhang Tonghua |
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| Abstract: | The protective effect of silybin on the micro-model of Coxsackie B5 virus infected new born rat heart cell culture was observed. After inoculation with 400TCID Coxsackie B5 virus, the cardiac enzyme s-lactic acid dehydrogenase (LDH) and glutamic oxaloacctic transaminase (GOT) were much lower in the silybin-treated groups than in those groups of untreated, but the synthesis rate of DNA was higher. Virus titer in the supernatant of cultures was detected 96 h after virus challenge. The virus titer (Lg 3. 95 TCID50) of the silybin-treated was lower than in that of untreated groups (Lg 6. 95 TCID50). These results suggest that silybin may be a useful drug in treatment of acute viral myocarditis. |
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| Keywords: | cell culture Coxsackie virus silybin heart muscle |
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