Diagnostic value of fibronectin and mutant p53 in the urine of patients with bladder cancer: impact on clinicopathological features and disease recurrence |
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Authors: | Sanaa Eissa Samir F Zohny Abdel-Rahman N Zekri Tarek Mostafa El-Zayat Amany M Maher |
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Institution: | (1) Oncology Diagnostic Unit, Medical Biochemistry Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11566, Egypt;(2) Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt;(3) Virology and Immunology Unit, Cancer Biology Department, Cairo University, Gizza, Egypt;(4) Urology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt |
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Abstract: | Development of new methods for bladder cancer detection is required because cystoscopy is invasive, and voided urine cytology
(VUC) has low sensitivity. The aim of this study was to evaluate the diagnostic performance of urinary fibronectin and mutant
p53 in comparison with VUC in the detection of bladder cancer. This study included 100 patients diagnosed with bladder cancer,
93 patients with benign urological disorders and 47 healthy volunteers. The urine supernatant was used for determination of
fibronectin by ELISA, while urine sediment was used for cytology and detection of mutant p53 by PCR-SSCP followed by DNA sequencing.
The sensitivity and specificity were 59% and 91.4% for VUC, 82% and 84.3% for fibronectin, and 37% and 100% for mutant p53;
combination of the three parameters increased sensitivity to 95% but specificity was only 78.6%. A significant association
was observed between disease recurrence and mutant p53, stage and lymph node involvement. Our results indicate that fibronectin
had the highest sensitivity compared to VUC and mutant p53 in bladder cancer detection; however, mutant p53 had superior specificity
compared to VUC and fibronectin. Mutant p53 is associated with disease recurrence and hence it has a significant prognostic
role in bladder cancer. |
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