| 肠胃清对人大肠癌细胞HCT8／V皮下移植瘤长春新碱耐药的逆转作用 |
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| 引用本文： | 许建华,邓皖利,孙珏,张勇,李朝衡,范忠泽. 肠胃清对人大肠癌细胞HCT8／V皮下移植瘤长春新碱耐药的逆转作用[J]. 上海中医药大学学报, 2009, 0(2): 59-63 |
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| 作者姓名： | 许建华 邓皖利 孙珏 张勇 李朝衡 范忠泽 |
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| 作者单位： | 上海中医药大学附属普陀医院肿瘤科; |
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| 基金项目： | 国家自然科学基金资助项目(30672683);;上海市教委科研基金资助项目(05CZ16) |
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| 摘 要： | 目的：观察中药复方肠胃清口服液逆转大肠癌长春新碱耐药的作用，初步探讨其逆转耐药的作用机制。方法：建立人大肠癌耐长春新碱（VCR）细胞株HCT8／V裸鼠皮下移植瘤模型，随机分为正常对照组、VCR组、肠胃清组、肠胃清高剂量＋VCR组、肠胃清低剂量＋VCR组。治疗结束后计算瘤体抑制率；高效液相色谱法检测瘤内VCR药物浓度；流式细胞技术检测瘤内细胞周期和凋亡改变。结果：肠胃清高低剂量联合VCR的瘤体抑制率为47．47％、69．62％，明显高于单用肠胃清或VCR组；对照组VCR的含量为0．1745μg／g瘤重，而肠胃清高剂量联合VCR组的浓度为12．2236μg／g瘤重，是对照组的70．05倍；与对照组比较，药物干预后，G1期细胞明显增加，S期细胞明显低于对照组，细胞凋亡率明显增加，以肠胃清联合VCR组作用最强。结论：肠胃清逆转大肠癌移植瘤对VCR的耐药与通过增加瘤体内药物浓度、诱导细晌凋亡有关。
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| 关 键 词： | 肠胃清 大肠癌 多药耐药 长春新碱 皮下移植瘤 |
Reversal Effect of "Changweiqing Fluid" on Vincristine-risistance in Human Colorectal Cancer Cell Line HCT-8/V Tumor Xenograft Mice |
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| XU Jian-hua DENG Wan-li SUN Jue ZHANG Yong LI Chao-heng FAN Zhong-ze Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine. Reversal Effect of "Changweiqing Fluid" on Vincristine-risistance in Human Colorectal Cancer Cell Line HCT-8/V Tumor Xenograft Mice[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2009, 0(2): 59-63 |
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| Authors: | XU Jian-hua DENG Wan-li SUN Jue ZHANG Yong LI Chao-heng FAN Zhong-ze Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine |
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| Affiliation: | (Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine) |
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| Abstract: | Objective:To investigate the reversal effect of "Changweiqing Fluid"(CWQ)on multidrug resistance in human colorectal cancer cell and its mechanisms.Methods:Establish xenograft model of the vincristine(VCR)-risistant human colorectal cancer cell line HCT-8/V in nude mice.Then the mice were randomly divided into five groups:control group(NS),CWQ group,VCR group,lower dose CWQ + VCR group and higher dose CWQ+ VCR group.After treatment,the inhibition rate of tumor in each group were calculated.Intracellular VCR... |
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| Keywords: | Changweiqing（CWQ） coloreetal cancer multidrug resistance（MDR） vincristine （VCR） HCT-8/V tumor xenograft model |
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