| HPLC同时测定余甘子叶中槲皮素和山奈酚的含量 |
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| 引用本文： | 毛健,刘振杰.HPLC同时测定余甘子叶中槲皮素和山奈酚的含量[J].中国当代医药,2014,21(19):50-52. |
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| 作者姓名： | 毛健 刘振杰 |
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| 作者单位： | 毛健 (广西壮族自治区富川县人民医院临床药学科,广西富川,542700)； 刘振杰 (广西壮族自治区药用植物研究所保育中心,南宁,530023)； |
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| 摘 要： | 目的建立HPLC同时测定余甘子叶中槲皮素和山奈酚含量的方法。方法色谱柱：Thermo Hypersil BDS C18（4.6 mm×250 mm,5μm）,梯度洗脱流动相：乙腈-0.1%磷酸溶液,流速：1.0 ml/min,检测波长：370 nm,柱温：30℃,进样体积：20μl。结果槲皮素和山奈酚分别在0.003~0.06μg/μl（r=0.9995）、0.001015~0.0203μg/μl（r=0.9993）范围内呈良好的线性关系,平均回收率（n=6）分别为98.83%（RSD=2.73%）、101.58%（RSD=2.21%）。结论建立了同时测定槲皮素和山奈酚含量的方法,可进一步完善余甘子叶的质量控制体系。
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| 关 键 词： | HPLC 余甘子叶 槲皮素 山奈酚 |
Simultaneous measurement of quercetin content and kaempferol content in Phyllanthus emblica leaf based on HPLC |
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| Institution: | MAO Jian ,LIU Zhen-jie( 1.Department of Clinical Pharmacy,People＇s Hospital of Fuchuan County in Guangxi Zhuang Autonomous Region, Fuchuan 542700,China;2.Child Care Center,Institute of Medicinal Plant Development in Guangxi Zhuang Autonomous Region,Nanning 530023,China) |
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| Abstract: | Objective To estsblish the HPLC method for simultaneous measurement of quercetin content and kaempfer- ol content in Phyllanthus emblica leaf. Methods Chromatographic column：Thermo Hypersil BDS C18（4.6 mm×250 mm, 5 μm）;gradient elution mobile phase：acetonitrile-0.1% phosphoric acid solution;flow rate：1.0 ml/min;measurement wavelength：370 nm;column temperature：30℃;injection volume：20 μl. Results Quercetin and kaempferol had good linear relation within tlae range of 0.003-0.06 μg/μl（r=0.9995） and 0.001015-0.0203 μg/μl（r=0.9993） respectively,with average recovery rate（n=6） of 98.83%（RSD=2.73%） and lO1.58%（RSD=2.21%） respectively. Conclusion The study is established the method for simultaneous measurement of quercetin content and kaempferol content,which helps further improvement of the quality control system for Phyllanthus emblica leaf. |
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| Keywords: | HPLC Phyllanthus emblica leaf Quercetin Kaempferol |
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