| 万古霉素对金黄色葡萄球菌生物膜黏附能力的影响 |
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| 引用本文： | 黄强,费军,余洪俊,黄显凯.万古霉素对金黄色葡萄球菌生物膜黏附能力的影响[J].创伤外科杂志,2011,13(6):489-493. |
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| 作者姓名： | 黄强 费军 余洪俊 黄显凯 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所全军战创伤中心,重庆,400042 |
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| 基金项目： | 国家自然科学基金资助项目，重庆市科技攻关项目 |
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| 摘 要： | 目的 探讨万古霉素对金黄色葡萄球菌形成生物膜早期黏附能力的影响.方法 孵育金葡菌在玻片上形成生物膜,对玻片上形成的细菌胞外多糖(extracellular polymeric substances,EPS)用荧光增白剂染色、黏附的细菌用SYTO9 /PI染色,再用激光共聚焦显微镜观察结合Image-Pro Plus V...
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| 关 键 词： | 生物膜 金黄色葡萄球菌 万古霉素 |
Effect of Vancomycin on adhesion of the staphylococcus aureus |
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| Institution: | HUANG Qiang,FEI Jun,YU Hong-jun,et al. (War Wound Centre,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To investigate the effects of Vancomycin on the adhesion ability of the staphylococcus aureus.Methods Staphylococcus aureus were grown to biofilms on the glass.The extracellular polymeric substances(EPS) of staphylococcus aureus was stained with the fluorescent whitening agent,the adherent bacteria was stained with SYTO9 /PI.The laser scanning confocal microscope in combination with Image-Pro Plus Version 6.0 software was used to perform the quantitative analysis of the EPS and the bacterial colony.Results Six hours after adding Vancomycin,the area and IOD value of EPS of 1/2MIC(minimum inhibitory concentration),1MIC,2MIC and 4MIC were 2183.33±923.90 and 3510.56±1482.10、3364.00±1885.30 and 5400.09±3026.40、2998.33±427.70 and 4815.24±689.10、3908.33±2030.70 and 6273.83±3259.90 respectively.They were all significantly increased after intervention with vancomycin.The bacteria in the EPS increased and colony closed up after administration of 1/2MIC,1MIC and 2MIC concentration(P<0.05).Conclusion The EPS and the bacteria increased after intervention with Vancomycin.Vancomycin can increase the initial adhesion ability of staphylococcus aureus. |
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| Keywords: | biofilm staphylococcus aureus Vancomycin |
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