兔延迟术后脂肪瓣内间充质干细胞增殖的实验研究

目的 探讨采用脂肪瓣延迟术等缺血缺氧的方法,促使脂肪来源的兔间充质干细胞(ASCs)增殖的可能性.方法 以兔为动物模型,在一侧腹股沟处形成脂肪瓣.21 d后取出瓣内脂肪组织及对侧正常脂肪组织,消化过筛离心,收集离心后沉淀物中的细胞,用流式细胞仪检测细胞的表面标记,计算细胞CD29、CD44、CD14和CD45的表达率并进行组间比较.结果 脂肪瓣延迟术后,瓣内细胞CD29和CD44的表达率增加,分别为74.06%和90.74%,未经处理的对侧脂肪组织分别为62.88%和77.54%,差异有统计学意义(P<0.05);瓣内细胞CD14和CD45的表达率减少,分别为57.66%和4.84%,未经处理的对侧脂肪组织分别为72.10%和75.82%,差异有统计学意义(P<0.05或P<0.01).结论 脂肪瓣延迟术等缺血缺氧的方法,可促进ASCs的增殖,是用瓣内组织移植后取得更好效果的机制之一;对脂肪组织先进行缺血预处理再移植,可能为一个临床脂肪移植的新方法.

Proliferation of the mesenchymal stem cells in a delayed fat flap: an experimental study in rabbits

Objective To investigate the possibility to enhance the proliferation of adipose-derived stem cells (ASCs) in a delayed fat flap in rabbits. Methods A delayed fat flap was formed in one side of inguinal region of a rabbit. 21 days after operation, the fat tissues at the delayed flaps and at the unoperated side were harvested and digested with 0.25% collageusse and sieved. The cell suspensions were centrifuged. The cells were obtained from tissue precipitate after centrifugation. The expression rates of the surface marker (CD29 CD44,CD14 and CD45) were measured by FCM and compared between the experimental and control groups. Results Expression rates of CD29 and CD44 were higher in the delayed fat flap (74.06% and 90.74%) than in the eontralateral fat tissue (62.88% and 77.54%, P < 0.05), while those of CD14 and CD45 were lower in the delayed fat flap (57.66% and 4.84%) than in the eontralateral fat tissue (72.10% and 75.82%, P < 0.05 and P < 0.01). Conclusions Tissue hypoxic isehemia such as fat tissue in a delayed fat flap can promote proliferation of ASCs. It indicates that tissue in the delayed flap may be transplanted with better survival rate. The ischemia pretreatement of fat tissue may become a new method for fat transplantion.