| 叶酸和聚乙二醇双修饰的壳聚糖纳米粒的制备及其性能表征 |
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| 引用本文： | 谢黎崖,胡权,吴永良,柯金珍,詹传明,侯振清. 叶酸和聚乙二醇双修饰的壳聚糖纳米粒的制备及其性能表征[J]. 中国现代应用药学, 2013, 30(3): 284-289 |
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| 作者姓名： | 谢黎崖 胡权 吴永良 柯金珍 詹传明 侯振清 |
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| 作者单位： | 厦门大学附属第一医院，福建 厦门 361003;厦门大学生物医学工程研究中心，福建 厦门 361005;厦门大学附属第一医院，福建 厦门 361003;厦门大学附属第一医院，福建 厦门 361003;厦门大学生物医学工程研究中心，福建 厦门 361005;厦门大学生物医学工程研究中心，福建 厦门 361005 |
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| 基金项目： | 厦门市科学技术计划资助项目(3502Z20114007) |
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| 摘 要： | 目的 利用离子交联和化学交联相结合的方法制备壳聚糖纳米粒子(NPs)，并对NPs分别进行了叶酸(FA)和聚乙二醇(PEG)的修饰。方法 通过红外光谱进行结构验证；用扫描电镜和粒度分析仪对粒子的微观形态、粒径、电位等进行了表征；通过与Hela细胞摄取实验对其靶向作用进行验证。结果 离子交联和化学交联相结合的方法制备壳聚糖纳米粒子粒径在200 nm左右并且粒径分布窄，修饰后的NPs(FA-NPs、PEG-NPs及FA+PEG-NPs)粒径不受功能基团修饰的影响。激光共聚焦试验证明FA-NPs及FA+PEG-NPs能显著提高细胞对粒子的摄取，而PEG-NPs则明显降低其对粒子的摄取。结论 FA+PEG-NPs有望成为一种新型的药物载体，用于抗癌药物对癌细胞的主动靶向。
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| 关 键 词： | 药物释放系统；壳聚糖；叶酸；聚乙二醇；纳米载体 |
| 收稿时间： | 2012-06-28 |
| 修稿时间： | 2012-11-07 |
Preparation and Characterization of Folic Acid and PEG Conjugated Chitosan Nanoparticles |
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| XIE Liy,HU Quan,WU Yongliang,KE Jinzhen,ZHAN Chuanming and HOU Zhenqing. Preparation and Characterization of Folic Acid and PEG Conjugated Chitosan Nanoparticles[J]. The Chinese Journal of Modern Applied Pharmacy, 2013, 30(3): 284-289 |
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| Authors: | XIE Liy HU Quan WU Yongliang KE Jinzhen ZHAN Chuanming HOU Zhenqing |
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| Affiliation: | First Affiliated Hospital of Xiamen University, Xiamen 361003, China;Research Center of Biomedical Engineering of Xiamen University, Xiamen 361005, China;First Affiliated Hospital of Xiamen University, Xiamen 361003, China;First Affiliated Hospital of Xiamen University, Xiamen 361003, China;Research Center of Biomedical Engineering of Xiamen University, Xiamen 361005, China;Research Center of Biomedical Engineering of Xiamen University, Xiamen 361005, China |
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| Abstract: | OBJECTIVE An ionic gelation combined with chemical crosslinking method was developed to prepare chitosan nanoparticles, followed by conjugation with folate (FA) and polyethylene glycol (PEG). METHODS The structures were verified by infrared spectroscopy. The morphology, diameter and Zeta electric potential of the nanoparticles were assayed by environmental scanning electron microscope and scattering particle analyzer. The specificity of the FA+PEG-NPs targeting cancer cells was demonstrated by human adenocarcinoma Hela cells. RESULTS The chitosan NPs presented a narrow size distribution with an average diameter about 200 nm regardless of the type of functional group. Laser confocal scanning imaging proved that both FA+PEG-NPs and FA-NPs could greatly enhance uptake by Hela cells. However, the PEG-NPs showed contrary results. CONCLUSION FA+PEG-NPs can be applied as a new vehicle to actively deliver anticancer drugs to tumor cells. |
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| Keywords: | drug release system chitosan folic acid PEG nanocarriers |
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