双黄连粉针剂对小鼠T淋巴细胞体外活化与增殖的影响

目的:探讨双黄连粉针剂(以下简称SHL)对小鼠T淋巴细胞的体外活化和增殖的影响.方法:MTT法检测SHL对小鼠淋巴细胞的毒副作用;双色荧光抗体染色技术结合流式细胞术分析SHL对小鼠T淋巴细胞在多克隆刺激剂(ConA)刺激下体外活化抗原CD69表达的影响;MTT法以及羧基荧光素乙酰乙酸(CFDA-SE)标记技术结合流式细胞术分析SHL对小鼠T淋巴细胞在ConA诱导下体外增殖的影响.结果:SHL对小鼠淋巴结来源的淋巴细胞毒副作用非常小;终质量浓度为60、 80、 100、 120 mg/L的SHL对ConA刺激下的T淋巴细胞体外活化抗原CD69表达具有抑制作用(P<0.01);MTT法和CFDA-SE染色法都显示, 终质量浓度为60、 80、 100、 120 mg/L的SHL对ConA诱导的T淋巴细胞增殖作用具有明显的抑制作用(P<0.01).结论:SHL对小鼠T淋巴细胞的体外活化和增殖具有明显的抑制作用.

Effect of shuanghuanglian injection on the activation and proliferation of T lymphocytes in mice in vitro

AIM: To study the effect of shuanghuanglian injection on the activation and proliferation of T lymphocytes in mice in vitro. METHODS: The toxic effect of SHL on lymphocytes in mice was estimated by MTT test; Double-fluorescent plus flow cytometry to analyze the effect of SHL on the activation of T lymphocytes in mice stimulated by ConA in vitro; MTT test and CFDA-SE plus flow cytometry were used to detect the effect of SHL on the proliferation of T lymphocytes in mice induced by ConA in vitro. RESULTS: SHL had little side effect on lymphocytes in mice in vitro; At the mass concentration of 60, 80, 100, 120 mg/L, SHL inhibited the activation of T lymphocytes in mice stimulated by ConA in vitro (P<0.01); Both MTT test and CFDA-SE stain sign that SHL inhibited the activation of T lymphocytes in mice induced by ConA in vitro at the mass concentration of 60, 80, 100, 120 mg/L (P<0.01). CONCLUSION: SHL can inhibit the activation and proliferation of T lymphocytes in mice in vitro.