| siRNA对K562细胞株bcr-abl融合基因表达的抑制 |
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| 引用本文： | 蒋磊,吴建波,俞康,倪吴花.siRNA对K562细胞株bcr-abl融合基因表达的抑制[J].中国实验血液学杂志,2004,12(3):332-334. |
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| 作者姓名： | 蒋磊 吴建波 俞康 倪吴花 |
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| 作者单位： | 1. 温州医学院附属第一医院科学研究所
2. 温州医学院附属第一医院血液科,温州,325000 |
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| 基金项目： | 浙江医药卫生优秀青年基金资助项目,编号SYQ0 0 0 02 |
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| 摘 要： | 为了探索CML的治疗新思路研究了siRNA(small interfering RNA)对K562细胞株bcr-abl融合基因表达的抑制作用。制备特异性对应bcr-abl融合基因的siRNA，转染K562细胞株，采用RT-PCR法测定bcr-abl融合基因mRNA的表达。结果表明：siRNA对bcr-abl基因表达的抑制作用随浓度的增加而增强，0.2ug siRNA能使bcr-abl融合基因mRNA的表达在24和48小时分别降低至对照组的19.9％和26.6％，但72小时时恢复到原水平，同时转染siRNA后细胞生长受到抑制。结论：siRNA可以有效的抑制K562细胞株中bcr-abl融合基因的表达。
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| 关 键 词： | siRNA bcr-abl融合基因 K562细胞 |
| 文章编号： | 1009-2137(2004)03-0332-03 |
| 修稿时间： | 2003年8月25日 |
Inhibitory Effect of siRNA on bcr-abl Gene Expression in K562 Cell Line |
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| JIANG Lei,WU Jian-Bo,YU Kang,NI Wu-Hua Institute of Medical Sciences and.Inhibitory Effect of siRNA on bcr-abl Gene Expression in K562 Cell Line[J].Journal of Experimental Hematology,2004,12(3):332-334. |
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| Authors: | JIANG Lei WU Jian-Bo YU Kang NI Wu-Hua Institute of Medical Sciences and |
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| Institution: | Institute of Medical Sciences, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. jianglei0299@hotmail.com |
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| Abstract: | To explore a new way to treat CML, inhibitory effect of small interfering RNA (SiRNA) on bcr-abl fusion gene expression of K562 cell line was studied. SiRNA for bcr-abl gene was designed and transfected into K562 cells, bcr-abl gene expression was tested by RT-PCR. The results showed that bcr-abl gene expression was inhibited by using siRNA in dose-dependent manner and reduced to 19.9% and 26.6% of the control at 24 and 48 hours after transfection with 0.2 micro g siRNA respectively. K562 cells proliferation was suppressed finally, but bcr-abl gene expression restored at 72 hours. In conclusion, anti-bcr-abl siRNA can effectively inhibit bcr-abl gene expression of K562 cell line. |
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| Keywords: | siRNA bcr-abl fusion gene K562 cell |
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