基于食管癌比较基因组杂交资料构建其进化树模型

［摘要］目的： 基于食管癌比较基因组杂交资料构建食管癌的树模型，探讨食管癌多基因参与、多途径发展、多步骤演进的发生发展规律。方法：根据收集的78例食管癌完整比较基因组杂交资料，利用Desper等建立的肿瘤树模型软件平台，构建了食管癌的树模型。 结果： 在78例食管癌病人构建的树模型中，有9个非随机的染色体扩增或者缺失事件被选取，即-4p、 -9p、 -18q、 +7p、 +8q、 +17p、 +17q、 +20p、 +20q等9个非随机事件，+7p、+8q和+20q很可能是食管癌发生发展过程中的早期事件；这些扩增或丢失的染色体上可能存在与食管癌发生、发展演进密切相关的瘤基因或者抑瘤基因。结论： 基于比较基因组杂交资料构建的食管癌树模型，提示食管癌多基因参与、多途径、多阶段发展演进模式，也为后续食管癌相关基因的探索指明了方向。

Construction of evolutionary tree model for esophageal carcinogenesis based on comparative genome hybridization data

AIM: Based on comparative genomic hybridization (CGH) data, to construct tree model of esophageal carcinoma and to explore mechanism of multigene involved, multistep development and multipathway progression during esophageal carcinogenesis. METHODS: Using the software developed by Desper et al, tree models of esophageal carcinoma were constructed according to the CGH data of 78 esophageal carcinoma patients. RESULTS: Tree models for esophageal carcinoma suggested that there were -4p, -9p, -18q, +7p, +8q, +17p, +17q, +20p, +20q nine nonrandom genetic events, and +7p、+8q and +20q might be important early events in esophageal carcinogenesis, indicating that there might be cancer-related genes in these chromosomal arms. CONCLUSION: Tree models based on CGH data of esophageal carcinoma imply the process of multigene involved, multistep and multipathway progression. The tree models also give the direction to search for esophageal cancer-related genes.