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| 肺癌新肿瘤标志物periplakin的筛选研究(英文) | |
| 引用本文： | Shuqin Dai,Wei Li,Mian Kong,Yuzhen Zheng,Shuying Chen,Junye Wang,Linquan Zang. 肺癌新肿瘤标志物periplakin的筛选研究(英文)[J]. 中德临床肿瘤学杂志, 2013, 12(7): 305-309. DOI: 10.1007/s10330-013-1178-y |
| 作者姓名： | Shuqin Dai Wei Li Mian Kong Yuzhen Zheng Shuying Chen Junye Wang Linquan Zang |
| 作者单位： | Department of Clinical Laboratory,Sun Yat-sen University Cancer Center;State Key Laboratory of Oncology in Southern China;Department of Thoracic Surgery,Sun Yat-sen University Cancer Center;Department of Pharmacology,College of Pharmacy,Guangdong Pharmaceutical University |
| 基金项目： | grants,from,the,Science,and,Technology,Planning,Project,of,Guangdong,Province(项目编号：2010B031600066,,2010B031500034,2008B030303008)the,Key,Scientific,Subject,Foundation,of,Ministry,of,Education,of,China(项目编号：208105)the,NationalScience,and,Technology,Major,Projects,for,New,Drugs(项目编号：2011zx09102-001-31) |
| 摘 要： | Objective: The aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer. Methods: Artificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR. Results: It was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer. Conclusion: Use specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed. |
| 关 键 词： | cDNA library lung cancer peptide phage display biomarker |
Screening study on new tumor marker periplakin for lung cancer |
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| Shuqin Dai,Wei Li,Mian Kong,Yuzhen Zheng,Shuying Chen,Junye Wang,Linquan Zang. Screening study on new tumor marker periplakin for lung cancer[J]. The Chinese-German Journal of Clinical Oncology, 2013, 12(7): 305-309. DOI: 10.1007/s10330-013-1178-y | |
| Authors: | Shuqin Dai Wei Li Mian Kong Yuzhen Zheng Shuying Chen Junye Wang Linquan Zang |
| Affiliation: | 11178. Department of Clinical Laboratory, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China 21178. State Key Laboratory of Oncology in Southern China, Guangzhou, 510060, China 31178. Department of Thoracic Surgery, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China 41178. Department of Pharmacology, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China |
| Abstract: | ObjectiveThe aim of this study was to use lung cancer targeting binding polypeptide ZS-9 to screen cDNA library of human lung cancer and obtain ZS-9 specific ligand to confirm tumor marker of non small-cell lung cancer.MethodsArtificially synthesize biotin labeled peptide ZS-9, anchored ZS-9 in the enzyme label plate coupled by avidin, used ZS-9 as probe to screen cDNA library of human lung cancer, after screening, obtained bacteriophage clone specifically binding with anchored polypeptide ZS-9. Extracted plasmid of bacteriophage and performed sequencing after amplified by PCR.ResultsIt was demonstrated by bioinformatic analysis on the sequence of ligand binded by lung cancer specific peptide ZS-9 that the ligand was the cytoskeletal protein periplakin on the surface of lung cancer cells, suggesting that periplakin might be a new marker for non-small-cell lung cancer in lung cancer.ConclusionUse specific lung cancer binding peptide to screen new tumor marker periplakin in lung cancer and further studies on its biologic functions in genesis and development of lung cancer are still needed. |
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