黄芪注射液对体外培养嗅鞘细胞增殖及其分泌神经营养因子的影响

背景:单独黄芪注射或嗅鞘细胞移植均可促进多种神经元的存活及脊髓损伤后神经功能的恢复,若联合嗅鞘细胞移植和中药黄芪注射液治疗脊髓损伤,是否可达到更好临床效果?目的:观察黄芪注射液对嗅鞘细胞增埴及其分泌神经营养因子的影响.方法:分离培养新生24 h内SD大鼠嗅鞘细胞并纯化,采用免疫组织化学方法鉴定.取培养至第8天的嗅鞘细胞,种植于多聚赖氨酸包被的96孔板,置于37℃、体积分数5%CO2培养箱饥饿24 h后,加入2mg/L,20mg/L,200mg/L,2 g/L,20 g/L黄茛注射液作用24 h,采用MTT法和流式细胞仪检测黄芪注射液对嗅鞘细胞增殖的影响:采用ELISA法测定培养上清中胶质细胞源性神经营养因子的水平.以血清培养液组为阴性对照.结果与结论:与阴性对照组比较,2,20 mg/L质量浓度黄芪注射液可明显促进嗅鞘细胞增殖(P＜0.05,P＜0.01):不同质量浓度黄芪注射液均增加嗅鞘细胞G1细胞比例,减少S、G2期细胞所占比例,但差异无显著性意义(P＞0.05),但各质量浓度黄芪注射液均显著减少嗅鞘细胞凋亡数量(P＜0.05):2 mg/L质量浓度黄芪能对嗅鞘细胞分泌胶质细胞源性神经营养因子有显著的促进作用.结果说明黄芪注射液可协同嗅鞘细胞促进脊髓损伤的恢复.

Influence of astragalus injection on in vitro cultured olfactory ensheathing cell proliferation and neurotrophic factor secretion

BACKGROUND:Astragalus or olfactory ensheathing cell(OECs) transplantation alone can promote survival of various neurons and functional improvement of injured spinal cord.The clinical effect of astragalus in combination with OECs transplantation remains unclear.OBJECTIVE:To observe the effect of astragalus on the proliferation of OECs and the secretion of neurotrophic factors.METHODS:OECs of SD rats,24 hours old,were isolated and cultured,and identified by immunohistochemistry.OECs at culture day 8 were seed...