下丘脑内基因转染对大鼠癫痫行为的影响

目的探讨下丘脑过度兴奋对于颞叶癫痫行为学变化的影响，从而进一步阐明下丘脑与颢叶癫痫的关系以及谷氨酸受体2亚基Q型(glutamate receptor 2Q，GluR2Q)在癫痫发病中的作用机制。方法20只Wistar大鼠随机分为海人酸(kainic acid or kainite，KA)组(KA对照组)与KA GluR2Q组，分别观察两组大鼠的癫痫行为。结果KA对照组大鼠癫痫发作程度较轻，主要以部分性发作为主且发作次数少，持续时间短，较少出现全面性发作。KA GluR2Q组大鼠癫痫发作程度剧烈，部分性癫痫发作较KA对照组更早、更频繁且由部分性发作转化为全面性发作的比率高于KA对照组。结论通过HVJ-脂质体基因转染技术将GluR2Q基因转染到下丘脑乳头体可以提高其兴奋性，并使该兴奋性冲动通过下丘脑与海马之间的联络纤维传导至海马齿状回及CA3、CA1区，使海马区原有的兴奋性加强，表现为癫痫行为的加重，从而促进了癫痫的发展及传播。

The effect of gene transfection in hypothalamus on epileptic behavior of rats

Objective To study the influence of hyperexcitability of hypothalamus on the changes of temporal lobe epileptic behavior, and elucidate the relationship between hypothalamus and temporal lobe epilepsy, and the mechanism of glutamate receptor 2Q (GLuR2Q) in seizure pathogenesis. Methods Twenty Wistar rats were randomly divided into two groups: kainic acid (KA) group (control group), the rats were injected kainic acid into amygdala; and KA+GluR2Q group (experimental group), GluR2Q cDNA was transfected into supramammillary nuclei of hypothalamus by means of HVJ-liposome method and followed by injection of KA into amygdala in 48 h. Then epileptic behavior of rats in two groups was observed subsequently. Results Rats in KA group mainly presented focal seizures with moderate and fewer attacks, short duration, and seldom of generalized onsets. But compared with KA group, the rats in KA+GluR2Q group presented focal seizures much earlier and more frequently with severe attacks, and transformed from focal seizures into the generalized seizures much more often. Conclusion The excitability of hypothalamus can be elevated by transfection the GluR2Q gene into supramammillary nuclei of hypothalamus with HVJ-liposome transfection technique. The excitative impulses will be conducted to the dentate gyrus and CA3/CA1 areas through the association fiber between hypothalamus and hippocampus, and results in enhancing the intrinsic excitability of hippocampus. Then the aggravation of epileptic behavior is manifested and the development and transmission of epilepsy is promoted.