Nitric oxide enhances expression of raf kinase inhibitor protein in keratinocytes期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Nitric oxide enhances expression of raf kinase inhibitor protein in keratinocytes

Authors:	Der-An Tsao Hsin-Su Yu Huoy-Rou Chang

Affiliation:	Department of Medical Technology, Fooyin University, Kaohsiung county;; Department of Dermatology, Kaohsiung Medical University, Kaohsiung city;; Department of Biomedical Engineering, I-Shou University, Kaohsiung county, Taiwan

Abstract:	Abstract: Several reports have focused on the potential of nitric oxide (NO) to influence the proliferation and differentiation cascade in a number of mammalian cells. The purpose of this study was to determine the relationship between expression of raf kinase inhibitor protein (RKIP) and proliferation in keratinocyte with NO treatment. Normal human keratinocytes were treated with SNAP (NO donor) doses of 10⁻⁷, 10⁻⁶, 10⁻⁵, 10⁻⁴ and 0 m (control group) separately. Expression of protein and mRNA of RKIP, cell proliferation and apoptosis have been measured. These results showed that elevated expression of RKIP in keratinocyte with NO treatment may contribute to the pathological and physiological features of NO-inhibited proliferation.

Keywords:	apoptosis keratinocyte nitric oxide proliferation raf kinase inhibitor protein