| pEGFP-N1-PEDF重组质粒的构建和鉴定 |
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| 引用本文： | 李春明,张颖鹏,刘志刚,谭艳平.pEGFP-N1-PEDF重组质粒的构建和鉴定[J].江西医药,2013(12):1111-1112. |
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| 作者姓名： | 李春明 张颖鹏 刘志刚 谭艳平 |
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| 作者单位： | 南昌大学第二附属医院皮肤科,南昌330006 |
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| 摘 要： | 目的：构建 pEGFP-N1-PEDF真核表达质粒，并进行鉴定。方法利用RT-PCR方法从组织中提取PEDF的基因片段，与pMD18-T载体连接，构建pMD18-T-PEDF重组质粒，酶切后与pEGFP-N1真核表达载体连接，构建pEGFP-N1-PEDF真核表达质粒，进行测序、酶切鉴定，表明质粒构建成功。结果本实验成功构建了pEGFP-N1-PEDF真核表达质粒。结论为进一步研究利用PEDF基因治疗皮肤肿瘤提供实验基础。
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| 关 键 词： | pEGFP—N1 PEDF 基因 |
Construction and identification of eukaryotic expression vector pEGFP-N1-PEDF |
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| Institution: | LI Chunming , ZHA NG Y ingpeng ,LIU Zhigang,et al.( Depantment of Dermatology,Second Affiliated Hospital of Nanchang University,Nanchang,30006.) |
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| Abstract: | Objective To construct an eukaryotic expression vector carrying PEDF gene. Methods PEDF gene was cloned from tissue by using RT-PCR method and was inserted into pMD18-T to construct pMD18-T-PEDF plasmid ,and to construct pEGFP-N1-EDF plasmid by using restriction enzyme.To identify the plasmid by sequencing and restriction enzyme. Results The eukaryotic expression vector pEGFP-N1-PEDF was constructed and identified. Conclusion It provides the base of apply the PEDF in treatment of skin tumor. |
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| Keywords: | pEGFP-N1 PEDF Gene |
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