| 黄芩醇提液对甲型流感病毒核蛋白基因表达的影响 |
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| 引用本文： | 李平,张清,孙坚.黄芩醇提液对甲型流感病毒核蛋白基因表达的影响[J].江西医药,2013(12):1128-1130. |
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| 作者姓名： | 李平 张清 孙坚 |
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| 作者单位： | 南昌大学第四附属医院呼吸与危重症医学科,南昌330003 |
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| 摘 要： | 目的：探讨黄芩醇提液对甲型流感病毒核蛋白（NP）的作用。方法本实验设HeLa细胞组、pcDNA3.1（＋）/empty组、pcDNA3.1（＋）/NP 组、TFSB 组，其中pcDNA3.1（＋）/empty 组、pcDNA3.1（＋）/NP 组分别通过瞬时转染将重组质粒 pcDNA3.1（＋）/empty、pcDNA3.1（＋）/NP转染到HeLa细胞中；黄芩醇提液组在将重组质粒pcDNA3.1（＋）/NP转染到HeLa细胞的同时使用黄芩醇提液进行药物干预。结果与真核重组质粒pcDNA3.1（＋）/NP组比较，黄芩醇提液组NP基因起始拷贝数具有统计学意义，P＜0.05。结论黄芩醇提液能够下调甲型流感病毒NP基因的表达。
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| 关 键 词： | 黄芩醇提液 核蛋白 抗流感病毒药 荧光定量RT-PCR |
Study of ethanol extracted Scutellaria on influenza A virus nucleoprotein |
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| LI Ping,ZHUANG Qing,SUN Jian.Study of ethanol extracted Scutellaria on influenza A virus nucleoprotein[J].Jiangxi Medical Journal,2013(12):1128-1130. |
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| Authors: | LI Ping ZHUANG Qing SUN Jian |
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| Institution: | . (Department of Respinatiry Medicine fourth Affiliated Hospital of Nanchang University ,Nanchang ,330006 , China) |
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| Abstract: | Objective To investigate the role of ethanol extracted Scutellaria on influenza A virus nucleoprotein and the molecular mechanism of skullcap anti-influenza. Methods The experimental set up HeLa cell group,pcDNA3.1 （＋）/empty group、pcDNA3.1（＋）/NP group,ethanol extracted Scutellaria group. pcDNA3.1（＋）/empty group and pcDNA3.1（＋）/NP group transient trans-fected pcDNA3.1 （＋）/empty and pcDNA3.1 （＋）/NP into HeLa cells,respectively; the ethanol extracted Scutellaria group not only transfected pcDNA3.1 （＋）/NP into HeLa cells,also interfere with ethanol extracted Scutellaria. Fluorescent quantitative RT-PCR measured the amount of nucleic acids. Results To compared with eukaryotic recombinant plasmid pcDNA3.1 （＋）/NP group,the start copy number of NP gene have statistically significant of ethanol extracted Scutellaria group.（P〈0.05）. Conclusion Ethanol extracted Scutellaria could down-regulate the expression of NP gene. |
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| Keywords: | Ethanol extracted Scutellaria NP protein Anti-influenza drugs Fluorescence quantitative RT-PCR |
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